Fall semester 2017
Last week you amplified a 1.3 kb DNA fragment from Arabidopsis TTG gene. In this week you will electrophorese the amplified DNA to confirm that the target region was indeed amplified, then you will clone the amplified DNA into a TOPO vector, introduce the recombinant vector into E. coli competent cells, propagate the transformed E. coli cells, and culture the cells on agar plates to isolate the cloned fragment. Please read Chapter 6 in detail and pay more attention to Figures 6-4 and 6-5.
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Lab 1 lecture.PDFLab 2 lecture.PDFLab 3 lecture.PDFLab 4 lecture.PDFLab 5 lecture.PDFLab 6 lecture.PDFLab 7 lecture.PDFLab 8 lecture.PDFLab 9 lecture.PDF Lab 10 lecture.PDFLab 11 lecture.PDFLab 12 lecture. PDFLab 13 lecture.PDFLab 14 lecture.PDFLab 15 lecture.PDF
To view the steps of cloning (the summary of Labs 4 to 9), click on the icon below.
Steps of Cloning.PDF