Spring Semester 2016

Lab 6

Last week you amplified a 1.3 kb DNA fragment from Arabidopsis TTG gene.  In this week you will electrophorese the amplified DNA to confirm that the target region was indeed amplified; then you will clone the amplified DNA into a TOPO vector, introduce the recombinant vector into E. coli competent cells, propagate the transformed E. coli cells, and culture the cells on agar plates to isolate the cloned fragment.  Please read Chapter 6 in detail and pay more attention to Figures 6-4 and 6-5. 

 

 

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