Spring Semester 2017

 

Exercise 8

In this week you will electrophorese last week's digested plasmids (see Figure 7-2 on page 39, and Figure 8-2 on page 42) in order to confirm that the size and the physical map of the fragment you cloned are consistent with published data.  Then you will denature the digested fragments and will do the Southern.  A transfer set up for Southern blotting is shown in Figures 8-1 and 8-3.

 

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