Spring Semester 2017
For this week please read Exercise 7 in detail. Liquid cultures from white and blue colonies (pages 37 and 38) have been prepared for you; you will extract plasmid DNA from these cultures (pages 38 and 39) and then you will digest the extracted DNA with EcoRI and HindIII (pages 39 and 40) in order to confirm that the restriction map of the fragment you cloned last week is consistent with published data.
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Lab 1 lecture.PDF Lab 2 lecture.PDF Lab 3 lecture.PDF Lab 4 lecture.PDFLab 5 lecture.PDFLab 6 lecture.PDFLab 7 lecture.PDFLab 8 lecture.PDFLab 9 lecture.PDFLab 10 lecture.PDFLab 11 lecture.PDFLab 12 lecture.PDFLab 13 lecture.PDFLab 14 lecture.PDFLab 15 lecture.PDF
To view the Steps of Cloning (summary of Labs 4 to 9), please click on the icon below.
Steps of Cloning.PDF