Fall Semester 2016

 

  

Exercise 7

For this week please read Exercise 7 in detail.  Liquid cultures from white and blue colonies (pages 37 and 38) have been prepared for you; you will extract plasmid DNA from these cultures (pages 38 and 39), then you will digest the extracted DNA with EcoRI and HindIII (pages 39 and 40) in order to confirm that the restriction map of the fragment you cloned last week is consistent with published data.

 

 

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To view the Steps of Cloning (summary of Labs 4 to 9), please click on the icon below.

 

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