Questions of the Week
Note: Questions of the week are given at the end of labs 1, 3, 5, 6, 7, 9, 10, 12, 13, 14
- Question of the Week No. 1: Mitosis
- Answer: Colchicine arrests mitosis at the metaphase stage, because by inhibiting microtubule polymerization, chromosomes cannot move to opposite poles; therefore the anaphase stage won't be formed.
- Additional information: Colchicine is used to produce polyploidy; this is because without polymerization of microtubules replicated chromosomes at the metaphase stage cannot separate at anaphase and do not migrate to the opposite poles.
3' GAGCTG 5'
3' CAGCTC 5'
3. The reverse primer binds to the 3' end of the target sequence on the upper strand.
The forward primer binds to the 3' end of the target sequence on the lower strand.
Question of the Week No. 4: Cloning 1
- a) Tetracycline; because the AmpR gene has been disrupted by the PstI cut; this will interrupt the synthesis of the ampicillin-resistance protein.
- Question of the week No. 5: Cloning 2
- All kanamycin-resistant transformants will die on ampicillin-rich medium. The reason is that the EcoRI-HindIII fragment cloned into the vector will disrupt the structure of the ampicillin-resistance gene.
- Question of the Week No. 7: Regulation of Gene Expression
- Type 2.
- The reporter protein is translationally fused with the protein of interest; i.e. the reporter protein will be detected in areas where the protein of the gene of interest is expressed.
- Question of the Week No. 8: Crossing Over
- Parental OOOOOOOO or OOOOOOOO
- Meiotic segregation without crossing over OOOOOOOO or OOOOOOOO
- Meiotic segregation with crossing over OOOOOOOO or OOOOOOOO or
- OOOOOOOO or OOOOOOOO
- Aberrants OOOOOOOO or the like arrangements
- Question of the Week No. 9: Complementation Test
- Question of the Week No. 10: SSLP Mapping