Subhashree Subramanyam

Research Associate

Department: Agronomy
Phone: 765.494.7849
Fax: 765.494.5105
Office: WSLR 234

Research Interests:

Plant-insect interactions focusing on mechanisms of plant resistance and insect virulence.

Insect resistance gene identification and development of molecular markers for marker assisted breeding    

Evaluation of transgenic resistance using native promoter elements and insecticidal proteins​ 


Traditional biology tools combined with latest molecular biology approaches are being put to use to decipher the molecular mechanisms in play during gene-for-gene interactions between wheat (Triticum aestivum) and its major dipteran insect pest, the Hessian fly (Mayetiola destructor). Functional  genomics and proteomic tools are being explored to provide molecular insights into plausible resistance and susceptibility mechanisms in play. The vast genetic resources available for the model grass genome and nonhost of Hessian fly, Brachypodium distachyon, are being utilized for functional characterization of candidate defense-response genes for development of molecular tools to complement native resistance genes to overcome the economic devastations caused by this insect pest.

Selected Publications:
amanyaS, Shreve JT, Nemacheck JA, Johnson AJ, Schemerhorn, B, Shukle RH, Williams CE (2018). Modulation of nonessential amino acid biosynthetic pathways in virulent Hessian fly larvae (Mayetiola destructor), feeding on susceptible host wheat (Triticum aestivum).J Insect Physiol 105: 54-63
Subramanyam S, Xiangye X, Schemerhorn B, Williams CE (2017). Physical and metabolic consequences of Hessian fly infestation are more severe on nonhost Brachypodium distachyon than on host-plant resistant wheat. Arthropod Plant Interact 11: 767-783
Jonhson A, Abdel Moneim, HM, Flanders KL, Reay-Jones FPF, Reisig D, Stuart JJ, Subramanyam S, Shukle RH, Schemerhorn BJ (2017). A novel, economical way to assess virulence in field populations of Hessian fly (Diptera: Cecidomyiidae) utilizing wheat resistance gene H13 as a model. J Econ Entomol 110: 1863-1868
Subramanyam S, Nemacheck JA, XiaoX, McDonald MH, Williams CE (2016). Targeted discovery of single-nucleotide polymorphisms in an unmarked wheat chromosomal region containing the Hessian fly resistance gene H33. Crop Sci 55: 1106-1114​
Subramanyam S, Sardesai N,Minocha SC, Zheng C, Shukle RH, Williams CE (2015). Hessian fly larval feeding triggers enhanced polyamine levels in susceptible but not resistant wheat. BMC Plant Biol 15:3
Zhao C, ….. Subramanyam S,….. (2015). A massive expansion of effector genes underlies gall-formation in the wheat pest Mayetiola destructorCurr Biol 25:613-620
Aggarwal R, Subramanyam S, ZhaoC, Chen M-S, Harris MO, Stuart JJ (2014). Avirulence effector discovery in a plant galling and plant parasitic arthropod, the Hessian fly (Maytiola destructor). PLoS ONE 9(6): e100958​
Subramanyam S, Zheng C, Shukle JT, Williams CE (2013). Hessian fly larva attack triggers elevated expression of disease resistance dirigent-like-protein-encoding gene, HfrDrd, in resistant wheat. Arthropod Plant Interact 7:389-402

Shreve JT, Shukle RH, Subramanyam S, Johnson AJ, Schemerhorn BJ, Williams CE, Stuart JJ (2013). A genome-wide survey of small interfering RNA and microRNA pathway genes in a galling insect. J Insect Physiol 59:367-376​

Shukle RH, Subramanyam S, Williams CE (2012). Effects of antinutrient proteins on Hessian fly (Diptera: Cecidomyiidate) larvae. J Insect Physiol 58:41-48
Williams CE, Nemacheck JA, Shukle JT, Subramanyam S, Saltzmann KD, Shukle RH (2011). Induced epidermal permeability modulates resistance and susceptibility of wheat seedlings to herbivory by Hessianfly larvae. J Exp Bot 4521-4531
Liu X, Williams CE, Nemacheck JA, Wang H, Subramanyam S, Zheng C, Chen, M-S (2010). Reactive oxygen species are involved in plant defense against a gall midge. Plant Physiol 152:985-999
Shukle RH, Subramanyam S, Saltzmann KA, Williams CE (2009). Ultrastructural changes in the midguts of Hessian fly larvae feeding on resistant wheat. J Insect Physiol 56: 754-760
manyam S,Smith DF, Clemens JC, Webb MA, Sardesai N, Williams CE (2008). Functional characterization of HFR1, a high-mannose N-glycan-specific wheat lectin induced by Hessian fly larvae. Plant Physiol 147:1412-1426
Subramanyam S,Sardesai N, Puthoff DP, Meyer JM, Nemacheck JA, Gonzalo M, Williams CE (2006). Expression of two wheat defense-response genes, Hfr-1 and Wci-1, under biotic and abiotic stresses. Plant Sci 170: 90-103
Sardesai N, Nemacheck JA, Subramanyam S, Williams CE (2005). Identification and mapping of H32, a new wheat gene conferring resistance to Hessian fly. Theor Appl Genet. 111:1167-1173
Sardesai N, Subramanyam S, Nemacheck JA, Williams CE (2005). Modulation of defense-response gene expression in wheat during Hessian fly larval feeding. J Plant Interact 1: 39-50
Puthoff DP, Sardesai N, Subramanyam S, Nemacheck JA, Williams CE (2005). Hfr-2, a wheat cytolytic toxin-like gene, is up-regulated by virulent Hessian fly larval feeding. Mol Plant Patol 6:411-423

Media Release:
Resistant wheat goes for the gut to protect against Hessian flies
Salivating over wheat plants may net Hessian flies big meal or death​