Research

Trait Genetics

United State SRWW Historical Panel: We have assembled >300 historical and contemporary SRWW accessions (named US Wheat History, USWH panel) by sampling from US genebank in Aberdeen, ID. This panel includes accessions grown in a long range of time from 200 years through recent releases of 2015 in United State. Therefore, this collection represents lines that were released before and after the introduction of height reducing (Rht) genes during the Green Revolution. This panel is unique for studying historical events such as loss and gains of favorable alleles using population genetics techniques and genotyping-by-sequencing data. We grew USWH panel in row-plots in ACRE and performed genomewide association studies for kernel weight. This study, identified several loci with implications in kernel weight. We also analyzed controlled-environment grown seedling root traits using roll-ups and WinRHIZO and were able to characterize phenotypic distribution and genetics of main root axis, root branching, and root dry matter.

Purdue Contemporary SRWW germplasm: We have assembled > 400 soft red winter wheat (SRWW) ever produced by the small grains breeding program over the last few decades. These are all crosses made after 1980 but the parental lines, themselves, are mainly from Purdue breeding program. They represent germplasm developed by elite x elite crosses. This Purdue panel is being genotyped using next-gen sequencing methods. Our objective is to characterize Fusarium head blight (FHB) resistance in this germplasm using field and greenhouse approaches. We will also characterize agronomic traits. A preliminary yield trial conducted for a subset of these materials to increase seed and evaluate winter hardiness is depicted below. 

Hyperspectral aerial image taken from Purdue panel preliminary trial 2017 by remote sensing team led by Dr. Melba Crawford.

Hyperspectral aerial image taken from Purdue panel preliminary trial 2017 by remote sensing team led by Dr. Melba Crawford.

Elite SRWW panel: We received the seed and genotype data for the SRWW elite panel from Dr. Sneller and Dr. Brown-Guedira, respectively. This is a publically available resource that was developed by NIFA-supported Triticeae CAP project. We are in the process of measuring traits beyond standard nursery traits. We identified grain yield QTL that were stable between the two replicates of 2017. 

Two layouts of 13 column x 24 rows were planted side-by-side in ACRE to evaluate grain yield and yield components. For grain yield marker trait associations were stably identified in the two replicates individually and in the average dataset.

Two layouts of 13 column x 24 rows were planted side-by-side in ACRE to evaluate grain yield and yield components. For grain yield marker trait associations were stably identified in the two replicates individually and in the average dataset.

 

Contact us if you think your research interest is in alignment with research described above and you are willing to join our laboratory.

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