Christopher Bidwell

 

Christopher Bidwell's Main Page

Research Program

Molecular Genetics

We are studying genes that regulate growth and reproduction in mammals and fish.

My laboratory uses the tools of molecular and cellular biology to study growth and reproduction in mammals and fish. We are working to identify and isolate genes that are directly involved in growth in mammals based on biochemical properties, tissue-specific expression, and chromosomal location. In fish, we are studying the genetics of polyploids and interspecific hybrids as well as genes involved in reproduction. We are working on both vitellogenin synthesis in the liver as well as receptor mediated uptake of the egg yolk proteins by the oocytes. Egg production in fish and poultry has potential application as an animal bioreactor system.

Research Techniques

  • Flow cytometry
  • Genomic DNA cloning
  • Polymerase chain reaction
  • Protein and DNA gel electrophoresis
  • DNA sequencing

Selected Publications

Blanton, J.R., A.L. Grant, D.C. McFarland, J.P. Robinson, and C.A. Bidwell. 1999. Isolation of two populations of myoblasts from porcine skeletal muscle. Muscle & Nerve 22, 43-50.

Houseknecht, K.L., C.A. Bidwell, C.P. Portocarrero, and M.E. Spurlock. 1998. Cloning and regulation of porcine peroxisome proliferator-activated receptor g . Gene 225, 89-96.

Spurlock M.E., M.A. Ranalletta, S.G. Cornelius, G.R. Frank, G.M. Willis, S.Q. Ji, A.L. Grant, and C.A. Bidwell. 1998. Leptin expression in porcine adipose tissue is not increased by endotoxin but is reduced by growth hormone. Journal of Interferon & Cytokine Research. 18(12), 1051-1058.

Reecy, J.M., C.A. Bidwell, O.A. Andrisani, D.E. Gerrard, and A.L. Grant. 1998. Multiple regions of the porcine a-skeletal actin gene control and modulate muscle specific expression in cell culture and directly injected skeletal muscle. Animal Biotechnology 9(2), 101-120.

Spurlock, M.E., G.R. Frank, S.G. Cornelius, S. Ji, G.M. Willis, and C.A. Bidwell. 1998. Obese gene expression in porcine adipose tissue: Relationship to fat mass and effect of energy intake restriction. Journal of Nutrition 128(4), 677-682.

Cao, H., C.A. Bidwell, S.K. Williams, and S.E. Mills. 1998. Rapid Communication: Nucleotide sequence of the gene for the porcine b1-adrenergic receptor. Journal of Animal Sciences 76(6), 1720-1721.

Bidwell, C.A., S. Ji, G.R. Frank, S.G. Cornelius, G.M. Willis, and M.E. Spurlock. 1997. Cloning and expression of the porcine obese gene. Animal Biotechnology 8, 191-206.

Liang, W., C.A. Bidwell, S. Williams, and S. Mills. 1997. Rapid Communication: Molecular cloning of the porcine b2-adrenergic receptor gene. Journal of Animal Sciences 75, 2824.

Reecy, J.M., C.A. Bidwell, G.P. Briley, and A.L. Grant. 1996. Structure and regulation of the porcine skeletal a-actin gene. Gene 180, 23-28.

Briley, G.P., P.K. Riggs, J.E. Womack, D.L. Hancock, and C.A. Bidwell. 1995. Chromosomal localization of the porcine skeletal muscle calpain gene. Mammalian Genome 7, 226-228.

Briley, G.P., J.M. Reecy, A.L. Grant, and C.A. Bidwell. 1995. Cloning and expression of the porcine myogenin gene. Animal Biotechnology 6, 79-92

Bidwell, C.A., and D.M. Carlson. 1995. Characterization of vitellogenin from white sturgeon Acipenser transmontanus. Journal of Molecular Evolution 41, 104-112.

Elkin, R.G., M.B. Freed, S.A.H. Danetz, and C.A. Bidwell. 1995. Proteolysis of Japanese quail and chicken plasma apolipoprotein B and vitellogenin by cathespin D: Similarity of resulting protein fragments with egg yolk polypeptides. Comparative Biochemistry and Physiology 112B, 191-196.

Briley, G.P., and C.A. Bidwell. 1994. Construction of a cosmid library using agarose embedded DNA. Biotechniques 17, 278-280

Blacklidge, K.H., and C.A. Bidwell. 1993. Three ploidy levels indicated by genome quantification in Acipenserformes of North America. Journal of Heredity 84, 427-430.

Blacklidge, K.H., and C.A. Bidwell. 1993. Laboratory fish egg incubator. Progressive Fish Culturalist 55, 204-206.

Riggs, P.K., T. Kuczek, C.L. Chrisman, and C.A. Bidwell. 1993. Analysis of aphidicolin-induced chromosome fragility in the domestic pig (Sus scrofa). Cytogenetics and Cell Genetics 62, 110-116.

Sun, W., S.Q. Ji, P.J. Ebert, C.A. Bidwell, and D.L. Hancock. 1993. Cloning partial cDNA's of m-calpain and m-calpain from porcine skeletal muscle. Biochimie 75, 931-936.

Bidwell, C.A., K. Kroll, E. Severud, S.I. Doroshov, and D.M. Carlson. 1991. Indentification and preliminary characterization of white sturgeon (Acipenser transmontanus) vitellogenin mRNA. General and Comparative Endocrinology 83, 415-424.

Bidwell, C.A., C.L. Chrisman, and G.S. Libey. 1985. Polyploidy induced by heat shock in channel catfish. Aquaculture 51, 25-32.