Zoltan Machaty

 

Zoltan Machaty's Main Page

Research Program

The overall objective of our laboratory is to improve reproductive efficiency in domestic animals by understanding the cellular and molecular mechanisms that regulate early embryonic development. Our primary goal is to define the signal transduction mechanisms and identify key signaling molecules that are involved in oocyte activation. Under normal conditions the fertilizing sperm activates the oocyte and stimulates its developmental program. Reproducing the events associated with sperm-induced oocyte activation is critical in a number of assisted reproductive technologies including nuclear transfer. Because nuclear transfer is currently the only technique available for the production of farm animals with targeted genetic modifications, major efforts are directed towards developing methods that would allow superior embryo development after nuclear transfer.

Research Techniques

  • Cell culture
  • Micromanipulation
  • Calcium imaging
  • Immunocytochemistry
  • PCR, RT-PCR

Selected Publications

Koh, S., Lee, K., Wang, C., Cabot, R.A., Machaty, Z. 2009. STIM1 regulates store-operated Ca2+ entry in oocytes. Dev. Biol. 330:368-376.

Xing, X., Magnani, L., Lee, K., Wang, C., Cabot, R.A., Machaty, Z. 2009. Gene expression and development of early pig embryos produced by serial nuclear transfer. Mol. Reprod. Dev. 76:555-563.

Lee K., Hyslop, J.M., Nanassy, L., Machaty, Z. 2009. Incidence of apoptosis in parthenogenetic porcine embryos generated by using protein kinase or protein synthesis inhibitors. Anim. Reprod. Sci. 112:261–272.

Pogranichniy, R., Lee, K., Machaty, Z. 2008. Detection of porcine parvovirus in the follicular fluid of abattoir pigs. J. Swine Health and Prod. 16:244-246.

Nanassy, L., Lee, K., Javor, A., Machaty, Z. 2008. Effects of activation methods and culture conditions on development of parthenogenetic porcine embryos. Anim. Reprod. Sci. 104:264–274.

Nanassy, L. Javor, A., Machaty, Z. 2007. Changes in MPF and MAPK activities in porcine oocytes activated by different methods. Theriogenology 68:146-152.

Vajta, G., Zhang, Y., Machaty, Z. 2007. Somatic cell nuclear transfer in pig: recent achievements and future possibilities. Reprod. Fertil. Dev. 19:403–423.

Lee, K., Fodor, W.L., Machaty, Z. 2007. Dynamics of lamin A/C in porcine nuclear transfer embryos. Mol. Reprod. Dev. 74:1221-1227.

Machaty, Z. 2006. Activation of oocytes after nuclear transfer. In: "Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis"; (Eds.: P.J. Verma and A.O. Trounson). Methods in Molecular Biology, Vol. 348. pp. 43. Humana Press, Totowa, NJ, USA.

Ramsoondar, J.J., Machaty, Z, Costa, C., Williams, B.L., Fodor, W.L., Bondioli, K.R. 2003. Production of a1,3-galactosyltransferase-knockout cloned pigs expressing human a1,2-fucosylosyltransferase. Biol. Reprod. 69:437-445.

Machaty, Z., Bondioli, K.R., Ramsoondar, J.J., Fodor, W.L. 2002. The use of nuclear transfer to produce transgenic pigs. Cloning & Stem Cells. 4:21-27.

Machaty, Z., Ramsoondar, J.J.,Bonk, A.J., Prather, R.S., Bondioli, K.R. 2002. Na+/Ca2+ exchanger in porcine oocytes. Biol. Reprod. 67:1133-1139.

Machaty, Z., Ramsoondar, J.J., Bonk, A.J., Bondioli, K.R., Prather, R.S. 2002. Capacitative calcium entry mechanism in porcine oocytes. Biol. Reprod. 66:667-674.