Postdoctoral Research Assistant
Hello, my name is Li Yang and I am a postdoctoral research assistant in Dr. Andy Tao’s group. I’m originally from Tianjin, China, where I received my B.S. degree from College of Pharmaceuticals and Biotechnology in Tianjin University. I came to US and continued my education under Dr. James Bruce’s instructions in Washington State University and I received my Ph.D. degree of Chemistry in 2011.
My Ph.D. research was focused on studying protein-protein interactions with novel cross-linking and mass spectrometry technologies. With this background of proteomics and protein interactions, I joined Dr. Tao’s group in 2011. My current project involves development of a mass spectrometry approach to trace PAMAM dendrimer delivery into live cells. Dendrimers are known as good drug transportation cargos; however the delivery mechanisms are still far away from being completely understood. Traditional methods, e.g. imaging methods can show the subcellular localizations of dendrimers. The new mass spectrometry approach will be used to reveal proteins that interact with dendeimers during the internalization process. The results of this project will complement the discoveries from the traditional imaging experiments, thus help understand the dendrimer delivery pathways. The new proteomics approach can be applied in studying not only dendrimers, but also other drug transportation vehicles in the future.
Outside the lab, I enjoy hiking, movies and spending time with my family and friends.
Yang, L.; Zheng, C.; Weisbrod, C. R.; Tang X.; Munske G. R.; Hoopmann, M. R.; Eng J. K.; Bruce, J. E., In Vivo Application of Photocleavable Protein Interaction Reporter Technology. J Proteome Res. 2012, 11(2), 1027-41.
Yang, L.*; Vaitheesvaran, B.*(co-first author); Hartil K.; Leroith D.; Robinson, A. J.; Hoopmann, M. R.; Eng, J. K.; Kurland, I.J.; Bruce, J. E., Fasted/Fed Mouse Metabolic Acetylome: N6-Acetylation Differences Suggest Acetylation Coordinates Organ-Specific Fuel Switching. J Proteome Res. 2011, 10(9), 4134-49.
Zheng, C.*; Yang, L.* (co-first author); Bruce, J. E., Cross-linking measurements of in vivo protein complex topologies. Mol Cell Proteomics. 2011, 10(10):M110.006841. Epub 2011 Jun 22.
Yang, L.; Tang, X.; Weisbrod, C. R.; Munske, G. R.; Eng, J. K.; von Haller, P. D.; Kaiser, N. K.; Bruce, J. E., A Photocleavable and Mass Spectrometry Identifiable Cross-linker for Protein Interaction Studies. Anal. Chem. 2010, (82), 3556-66. · Yang, L.; Zhang, H.; Bruce, J. E., Optimizing the Detergent Concentration Conditions for Immunoprecipitation (IP) Coupled with LC-MS/MS Identification of Interacting Proteins. Analyst 2009, 134(4), 755-62.
Vaitheesvaran, B; Yang, L.; Hartil, K.; Glaser, S.T.; Yazulla, S; Bruce, J. E.; Kurland,I.J., Peripheral effects of FAAH deficiency on fuel and energy homeostasis: Role of dysregulated lysine acetylation dysregulated lysine acetylation. PLoS One. 2012, 7(3):e33717. Epub 2012 Mar 19.
Zhang, H.; Tang, X.; Munske, G. R.; Zakharova, N.; Yang, L.; Zheng, C.; Wolff, M. A.; Tolic, N.; Anderson, G. A.; Shi, L.; Marshall. M. J.; Fredrickson, J. K.; Bruce, J. E. In Vivo Identification of the Outer Membrane Protein OmcA−MtrC Interaction Network in Shewanella oneidensis MR-1 Cells Using Novel Hydrophobic Chemical Cross-linkers. J. Proteome Res. 2008, 7(04), 1712-20.