I’m from Kaifeng, China and received my Ph.D. degree in January 2009 from Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) majoring in analytical chemistry. My Ph.D. research is on the selective isolation and separation of endogenous peptides and phosphopeptides from complex biological samples (such as serum) and also their application in the screening of bioactive compounds from traditional Chinese medicines (TCMs) and discovery of novel potential biomarkers for clinical diagnosis.
I joined the biochemistry department on March 2009 as a postdoctoral research assistant working in the laboratory of Dr. Andy Tao. My current research is focused on the preparation and application of dendrimer-based nanomedicine via mass spectrometry-based proteomic strategies. The highly branched and water soluble dendrimer has been widely explored in the fields of drug delivery, gene therapy due to its cells permeation ability. We functionalize the dendrimer with small drug molecules to seek specific drug targets through profiling small molecule–protein interaction. By probing the ligand binding partner we can discover unknown protein functions, identify the molecular mechanisms of drug action, and obtain information for optimization of the lead compounds. Our long objective is to deliver the dendrimer nanomedicine into living cells to probe protein targets in vivo, and finally elucidate the molecular signaling pathways and provide therapeutic agents for drug discovery.
EDUCATION03/2009-presentPostdoctoral Fellow (Tao’s Lab)Department of Biochemistry and Purdue Cancer CenterPurdue University, West Lafayette, IN
07/2004-01/2009Degree: Ph.D. (Advisor: Prof. Dr. Hanfa Zou)Key Laboratory of Separation Sciences for Analytical Chemistry,National Chromatographic R&A Center,Dalian Institute of Chemical Physics, Chinese Academy of Sciences
09/2003-07/2004Graduate’s Course StudyDepartment of ChemistryUniversity of Science & Technology of China, Hefei, China
09/1999-07/2003Degree: B.Sc.Department of ChemistryJilin University, Changchun, China
PUBLICATIONSLianghai Hu, Houjiang Zhou, Yinghua Li, Shutao Sun, Lihai Guo, Mingliang Ye, Xiaofeng Tian, Jianren Gu, Shengli Yang, Hanfa Zou. Profiling of endogenous serum phosphorylated peptides by titanium (Ⅳ) immobilized mesoporous silica particles enrichment and MALDI-TOF MS detection. Anal. Chem., 2009, 81: 94–104.
Lianghai Hu, Mingliang Ye, Hanfa Zou, Recent advances in mass spectrometry-based peptidome analysis. Expert Rev. Proteomics, 2009, 6(4): 433-447.
Lianghai Hu, Karl-Siegfried Boos, Mingliang Ye, Ren’an Wu, Hanfa Zou, Selective on-line serum peptide extraction and multidimensional separation by coupling a restricted access material-based capillary trap column with nanoLC-MS/MS. J. Chromatogr. A, 2009, 1216: 5377-5384.
Lianghai Hu, Xin Li, Xinning Jiang, Houjiang Zhou, Xiaogang Jiang, Liang Kong, Mingliang Ye, Hanfa Zou, Comprehensive peptidome analysis of mouse livers by size exclusion chromatography prefractionation and nanoLC-MS/MS identification. J. Proteome Res., 2007, 6: 801-808.
Lianghai Hu, Xin Li, Shun Feng, Liang Kong, Xingye Su, Xueguo Chen, Feng Qin, Mingliang Ye, Hanfa Zou, Comprehensive two-dimensional HPLC to study the interaction of multiple components in Rheum Palmatum L. with HSA by coupling a silica bonded HSA column to a silica monolithic ODS column. J. Sep. Sci., 2006, 29: 881-888.
Xingye Su, Lianghai Hu, Liang Kong, Xiaoyuan Lei and Hanfa Zou, Affinity Chromatography with Immobilized DNA Stationary Phase for Biological Fingerprinting Analysis of Traditional Chinese Medicines. J. Chromatogr. A, 2007, 1154: 132-137.
Ruijun Tian, He Zhang, Mingliang Ye, Xiaogang Jiang, Lianghai Hu, Xin Li, Xinhe Bao, Hanfa Zou, Selective Extraction of Peptides from Human Plasma by Highly Ordered Mesoporous Silica Particles for Peptidome Analysis. Angew. Chem. Int. Ed., 2007, 46: 962-965.
AWARDS:1. 2009 Eli Lilly Asia Outstanding Graduate Thesis Award2. 2007 BHP Billiton (Australia) Scholarship