Leonor Boavida

Botany and Plant Pathology

Assistant Professor

Phone

765.494.0031

Fax

765.494.0363

Office

WSLR Room 232

Email

lboavida@purdue.edu

Research Interests: Plant Reproductive Development, Gamete Identity and Function, Cell-Cell Communication

Dr. Boavida’s lab investigates the cellular and molecular mechanisms that regulate cell-cell recognition in plant fertilization. Fertilization is defined by the fusion of two haploid sex cells or gametes, the sperm and egg, which produce a diploid zygote that initiates a very sophisticated developmental program. However, this definition of fertilization does not capture the full scope of important cellular events that evolved as a hallmark of flowering plants: the occurrence of two separate gametic fusions (double fertilization). In addition to the sperm-egg cell fusion, a second sperm fuse with a sister female gamete, the central cell to produce the endosperm whose function is to nurture the developing embryo within the seed. This means that flowering plants likely evolved a new signal transduction machinery to ensure a robust recognition between male and female gametes. In this context is important that we address the following questions: which molecular factors mediate gamete interactions? Is there molecular specificity for the two distinct fusion events? Do these factors perform specific functions in recognition, activation, adhesion, and/or fusion of the gametes? What is the interplay between gamete recognition events and downstream signaling pathways leading to seed development? Are these factors, species–specific? We are now focused on the functions of scaffolding proteins and other transmembrane partners that have been recently identified as mediators of gamete interactions. Our aim is to build a comprehensive “fertilization interactome” that will allow us not only to have a better understanding of gamete factors and signaling pathways controlling plant gamete interactions but also to quiz the network concerning evolutionary aspects of the fertilization process and other important biological processes such as speciation, avoidance of inbreeding, hybridization or prevention of polyspermy.

Awards & Honors

(2014) Invited Scientist Grant. FCT - SFRH/BCC/52181/2013.

(2010) Post-Doctoral Grant. FCT - SFRH/BPD/43584/2008.

Selected Publications

Chagas Boavida, L. C., & Becker, J. D. (2016). Plant Evolution: What Does It Take To Be an Egg? Current Biology, 26, 527-530.

Jiang, H., Yi, J., Chagas Boavida, L. C., Chen, Y., Becker, J. D., Köhler, C., & McCormick, S. (2015). Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm. Proc Natl Acad Sci USA. doi:10.1073/pnas.1510854112

Chagas Boavida, L. C., Peng, Q., Broz, M., Becker, J. D., & McCormick, S. (2013). Arabidopsis Tetraspanins are confined to discrete expression domains and cell types in reproductive tissues and form homo- and heterodimers when expressed in yeast. Plant Physiology, 163, 696-712.

Borges, F., Gardner, R., Lopes, T., Calarco, J. P., Chagas Boavida, L. C., Slotkin, R. K., . . . Becker, J. D. (2012). FACS-based purification of Arabidopsis microspores, sperm cells and vegetative nuclei. BMC Plant Methods, 8-14.

Chagas Boavida, L. C., Borges, F. S., Becker, J. D., & Feijo, J. A. (2011). Whole-genome analysis of gene expression reveals coordinated activation of signaling and metabolic pathways during pollen-pistil interactions in Arabidopsis thaliana. Plant Physiology, 155, 2066-2080.

Cheung, A. Y., Chagas Boavida, L. C., Aggarwal, M., Wu, H. M., & Feijo, J. (2010). The pollen tube journey in the pistil and imaging the in vivo process by two-photon microscopy. J. Exp. Bot, 61, 1907-1915.

Chagas Boavida, L. C., & McCormick, S. (2007). Temperature as a determinant factor for increased and reproducible in vitro pollen germination in Arabidopsis thaliana. Plant Journal, 52, 570-582.