Innate Immunity in Maize
A key benefit of MAGIC is that it has the potential to reveal genetic variation that normally remains hidden or cryptic and therefore inaccessible to genetic dissection. By having a mutant in the background, MAGIC serves to hypersensitize the segregating populations to potential changes in the reporter phenotype, thereby reducing the background noise and amplifying the effect of the relevant gene/QTL in the trait of interest. This allows the detection and mapping of genes that have only subtle (though potentially important) effects in wild-type germplasm.
Mutant-Assisted Gene Identification and Characterization - (MAGIC)