Life Sciences Facilities
Purdue University research cores provide access for faculty, students and collaborators to state-of-the-art equipment and expertise to support basic and translational research in a broad range of scientific fields. Instrumentation, facilities, software and professional assistance are available for the design and conduct of specialized measurements, analysis and interpretation of data, and research collaboration.
The Analytical Mass Spectrometry Facility assists Purdue faculty in solving research problems by providing quality mass spectrometry data. The facility is equipped with a complete range of mass spectrometers, ionization methods, and sample introduction equipment in order to accommodate a broad array of sample types and scientific questions. A staff of expert mass spectrometrists enables the acquisition of informative data on rapid timescales.
1,408-acre field research station located seven miles northwest of campus. The studies range from basic to applied research including: plant breeding and genetics, crop production and soil tillage management, plant physiology, soil fertility, weed control, disease and insect resistance and control, and variety performance evaluation for corn, soybeans, small grains, sorghum and alfalfa.
An Herbarium is a collection of carefully preserved plant and/or fungal specimens; a Fungarium is a collection of carefully preserved fungal specimens. The Department of Botany and Plant Pathology at Purdue University houses two herbaria of immense historical and scientific value. The Arthur Fungarium (herbarium acronym PUR) is the largest specialized Fungarium, containing the most important collection of plant rust fungi (order Pucciniales), in the world. The Kriebel Herbarium (acronym PUL) contains non-rust fungi, vascular plants, lichens, algae and bryophytes, and is the oldest herbarium in the state of Indiana. Together the collections contain close to 250,000 specimens dating as far back as 1769 and gathered from across the globe. The collections are an important global resource for scientists and researchers in the fields of botany, mycology, plant pathology, forestry and biology. PUR and PUL arrange for loans of material to other herbaria and researchers around the world, accept material for deposition to voucher scientific studies, and serve as a resource for identifying rust fungi and their host plants from around the world.
The vision of the Bioinformatics Core is to facilitate, amplify, and accelerate biological research and discovery through application of bioinformatics. It will do so by delivering high quality analysis in a timely and economical manner. It will be responsive to customer needs and evolve with advances in the field. It will actively engage in and seek out opportunities to advance the educational mission of the University.
The Purdue Bioscience Imaging Facility (BIF) provides the instruments and expertise needed to visualize molecules in preparations ranging from single cells to entire animals. All facility users receive individualized instrument training as well as project-specific advice for optimal data acquisition. Consultation on sample preparation, image rendering and data analysis are also available as our knowledge base permits. Collaborations and contract research services are encouraged.
Computational Biology is one of the most important and exciting areas in all of science and technology, as it is positioned at the intersection of modern biology, quantitative modeling and high-performance computing. It focuses on the development and application of computational tools and techniques to solve complex problems in biosciences. Computational Biology helps provide a fundamental understanding of complex biological systems and offers the potential to significantly impact a wide variety of technologies, including drug discovery and novel therapies for human, animal and plant diseases.
The main focus of Computational Biology at the Bindley Bioscience Center is on experimental design and data analysis related to proteomics and metabolomics data applied to human health, animal health, and agricultural solutions.
Electron Microscopes use energetic electrons to image very small features on samples. Some have the capability to do micro-cutting with a Focused Ion Beam (FIB). The Electron Microscopy facility in Birck Nanotechnology Center has Hitachi S4800 cold field-emission scanning electron microscope, equipped with STEM capability; FEI Nova 200 Dual Beam system, equipped with a Klocke nanomanipulator for site specific sample preparation; and FEI Environmental S/TEM 80-300 Titan.
The Flow Cytometry and Cell Separation Facility in the Bindley Bioscience Center provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cell-cycle distribution, apoptosis measurements, cytokine detection, data analysis and more. We will train those users interested in running their own samples on the BD Fortessa or the BC FC500. Facility staff will run all experiments on the FACS Aria III. The facility provides expert consultation for protocol development so that experiments are appropriately designed and implemented.
The Purdue Genomics Core facility has more than a decade of experience in DNA sequencing and provides "next generation", high and low-throughput sequencing services as well as first-pass informatics support. The facility provides both next-generation Illumina HiSeq and MiSeq, "Sanger" sequencing and introducing and inexpensive next-generation sequencing service called "WideSeq."
The Life Science Microscopy Facility (LSMF) provides Purdue researchers access to state-of-the-art electron microscopy instrumentation and to provide service, consultation and training to assist scientists in achieving their research objectives. The LSMF is a full service electron microscopy core with both service and individual use options. Equipment includes two field-emission SEMs equipped with cryo, EDX, low vacuum, and tensile stage. Also available are 2 TEMs, routine and cryo sample preparation instrumentation, and other support equipment. Basic light microscopes are equipped with color digital cameras and optics for Bright-field, Nomarski DIC contrast, Fluorescence, and stereo imaging.
The Metabolite Profiling Facility (MPF) provides state-of-the-art technologies that enable both qualitative (defining all components of a metabolome) and quantitative (determining differential concentrations of metabolites) metabolomics in complex biological systems. This facility employs highly sensitive mass spectrometry coupled with liquid chromatography and multidimensional gas chromatography for precise sample analysis. Metabolite profiling is an integral component of systems biology, an exciting field that combines genomics, transcriptomics and proteomics to define cellular functionality. Our elite scientists empower researchers with new technologies, methods development, expert training and consultation.
The Purdue Cryo-EM Facility is a member of the Purdue EM Consortium and a designated Indiana CTSI Core Facility. Our facility is available to Purdue, other academic institutes, and industry users. It provides state-of-the-art instruments and expertise for high resolution structure determination of viruses, larger macromolecular complexes, nano-particles, as well as tomographic visualization of virus-cell interactions by cryo-electron microscopy (cryo-EM) and three-dimensional (3-D) image reconstruction.
The Purdue Proteomics Facility (PPF) provides innovative state-of-the-art LC-MS/MS analysis of proteins in clinical, environmental and other biological samples. As a shared facility of the Bindley Bioscience Center (BBC) and the Purdue University Center for Cancer Research, the facility enables both targeted and global analysis of proteins, protein post-translational modification studies, and the protein-protein interactions. Coupled with different chromatographic separation techniques, modern mass spectrometric instrumentations, and advanced scientific and bioinformatics expertise, the facility provides unique opportunities for researchers on and off Purdue campus to perform intact protein ("top-down") and peptide fragment ("bottom-up") analyses at a reasonable rates and reasonable turnaround time (5-10 days). Many projects employ these same technologies for biomarker discovery in health and disease — a signature research area at the BBC.