The overall objective of our laboratory is to improve reproductive efficiency in domestic animals by understanding the cellular and molecular mechanisms that regulate early embryonic development. Our primary goal is to define the signal transduction mechanisms and identify key signaling molecules that are involved in oocyte activation. Under normal conditions the fertilizing sperm activates the oocyte and stimulates its developmental program. Reproducing the events associated with sperm-induced oocyte activation is critical in a number of assisted reproductive technologies including nuclear transfer. Because nuclear transfer is currently the only technique available for the production of farm animals with targeted genetic modifications, major efforts are directed towards developing methods that would allow superior embryo development after nuclear transfer.
- Cell culture
- Calcium imaging
- PCR, RT-PCR
Gonzalez-Garcia JR, Bradley J, Nomikos M, Paul L, Machaty Z, Lai FA, Swann K. The dynamics of MAPK inactivation at fertilization in mouse eggs. J Cell Sci. 2014; 127(Pt 12):2749-60.
Lee K, Wang C, Spate L, Murphy CN, Prather RS, Machaty Z. Gynogenetic activation of porcine oocytes. Cell Reprogram. 2014; 16:121-9.
Wang C, Machaty Z. Calcium influx in mammalian eggs. Reproduction. 201; 145:R97-R105.
Machaty Z, Peippo J, Peter A. Production and manipulation of bovine embryos: techniques and terminology. Theriogenology. 2012; 78:937-50.
Gonzalez-Garcia JR, Machaty Z, Lai FA, Swann K. The dynamics of PKC-induced phosphorylation triggered by Ca2+ oscillations in mouse eggs. J Cell Physiol. 2013; 228:110-9.
Lee K, Wang C, Machaty Z. STIM1 is required for Ca2+ signaling during mammalian fertilization. Dev Biol. 2012; 367:154-62.
Wang C, Lee K, Gajdócsi E, Papp AB, Machaty Z. Orai1 mediates store-operated Ca2+ entry during fertilization in mammalian oocytes. Dev Biol. 2012; 365:414-23.
Lee K, Wang C, Chaille JM, Machaty Z. Effect of resveratrol on the development of porcine embryos produced in vitro. J Reprod Dev. 2010; 56:330-5.
Wang C, Swanson WF, Herrick JR, Lee K, Machaty Z. Analysis of cat oocyte activation methods for the generation of feline disease models by nuclear transfer. Reprod Biol Endocrinol. 2009; 7:148.
Koh S, Lee K, Wang C, Cabot RA, Machaty Z. STIM1 regulates store-operated Ca2+ entry in oocytes. Dev Biol. 2009; 330:368-76.