Bruce M Applegate

Department of Food Sciences

Professor of Food Science
Associate Professor Food Science

Phone

765.496.7920

applegab@purdue.edu

Curriculum Vitae

Education

B.A., Ph.D., University of Tennessee

Research

Detection of viable foodborne pathogens using bateriophage; automated extraction of nucleic acids from various matrices; enumeration of microorganisms (i.e. pathogens and other organisms) using quantitative PCR; the use of bioreporters in bioelectronics; metabolic engineering; detection of problematic microorganisms in industrial environments; construction of recombinant bacterial strains to rapidly evaluate antimicrobial products; microbial ecology.

Awards & Honors

(2009) NASA Inventions and Contributions Board Award. NASA.

(2005) Seed for Success Award. Vice Provost for Research.

Patents

Applegate, B. M., Morgan, M., Perry, 0., & Kothapalli, A. Methods for Generation of Reporter Phages and Immobilization of Active Bacteriophages on a Polymer Surface. U.S. Patent No. Filed Utility Patent # 12/549,500.. Washington, D.C.: U.S. Patent and Trademark Office.

Applegate, B. M. Bioluminescent biosensor device. U.S. Patent No. 6,544,729. Washington, D.C.: U.S. Patent and Trademark Office.

Selected Publications

Kim, S., Schuler, B., Terekhov, A., Auer, J., Mauer, L., & Perry, L. (2009). A bioluminescence-based assay for enumeration of lytic bacteriophage. Journal of Microbiological Methods, 79(1), 18-22.

Stratton, T., Garc, A., & Youngblood, J. (2009). Application of a High Throughput Bioluminescence-Based Method and Mathematical Model for the Quantitative Comparison of Polymer Microbicide Efficiency. Biomacromolecules, 10(5), 1173-1180.

Perry, L., SamMiguel, P., Minocha, U., Terekhov, A., Shroyer, M., Farris, L., . . . Reuhs, B. (2009). Sequence analysis of Escherichia coli O157:H7 bacteriophage FV10 and identification of a phage encoded immunity protein that modifies the O157 antigen. Fems Microbiology Letters, 292(2), 182-186.

Applegate, B. (2008). Chlorine Dioxide Oxidation of Dihydronicotinamide Adenine Dinucleotide (NADH) Inorganic Chemistry, 47, 2205-2211.

Habteselassie, M., Bischoff, M., Blume, E., Applegate, B., Reuhs, B., Brouder, S., & Turco, R. (2008). Environmental controls on the fate of Escherichia coli in soil. Water, Air and Soil Pollution, 190, 143-155.

Park, M., Banks, M., Applegate, B., & Webster, T. (2008). Influence of nanophase titania topography on bacterial attachment and metabolism. International Journal of Nanomedicine, 4(3), 8-Jan.

Applegate, B. (2008). Luminescence Techniques for the Detection of Bacterial Pathogens. In Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems (1, 213-230). Springer Science and Business Media.

Kim, S., Igboegwu, E., Terekhov, A., & Applegate, B. (2005). Bioluminescent Assay for Evaluating Bacteriophage Infectivity in a Food Model.

Hartano, L., Shroyer, M., Farris, L., Diefus-Dux, H., Turco, R., Reuhs, B., & Applegate, B. (2005). Development of an Escherichia coli O157:H7 Bioluminescent Reporter for Measuring Bioavailable Carbon.

Sedgley, C., Nagel, A., Hall, D., & Applegate, B. (2005). Influence of irrigant needle depth in removing bioluminescent bacteria inoculated into instrumented root canals using real-time imaging in vitro. International Endodontics Journal, 38(2), 97-104.