Bhunia Lab

 

                            

 Research    Research Group    Publications    Former Lab Members    Photo Gallery

 

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      Research Focus and Interests         

          Pathogen and Toxin Detection:

         · Biosensor technologies including laser light scattering, mammalian cell-based, and fiber optic sensors for rapid and high
         throughput  screening of live pathogens and toxins in food

· Development of biorecognition including antibodies, receptors, ligands, and microbiological growth media  for biosensor application
 
Host-Pathogen Interaction and Control Strategies Using Probiotics :

· Understanding the molecular and cellular mechanism of  Listeria monocytogenes colonization and translocation through epithelial barrier during intestinal phase of infection.

· Prevention and control using bioengineered probiotic and antimicrobial peptide loaded biocompatible nano-carrier.

 

 

  Our Collaborators

                Dr. Euiwon Bae , School of Mechanical Engineering, Purdue University

Dr. Rashid Bashir, University of Illinois at Urbana-Champaign

            Dr. Andrew Gehring, USDA-ARS, ERRC, Wyndmoor, PA

Dr. E. Dan Hirleman,  Dean, School of Engineering, University of California, Merced, CA
Dr. Mike Ladisch, Department of Agricultural & Biological Engineering, Purdue University
Dr. Albenones de Mesquita, Federal University of Goias, Goiania, Brazil
Dr. George Paoli, USDA-ARS, ERRC, Wyndmoor, PA
Dr. Yuan Yao, Department of Food Science, Purdue University

 

  Selected Publications

     Text Books
1. Foodborne Microbial Pathogens: Mechanisms and Pathogenesis, Arun K. Bhunia, 1st Edition (2008), Springer, New York.
2. Fundamental Food Microbiology, Bibek Ray and Arun K. Bhunia, 5th Edition (2014). CRC Press, Boca Raton, FL.

Refereed Publications
1. Singh, A.K., Bettasso, A.M., Bae, E., Rajwa, B., Dundar, M.M., Forstere, M.D., Liu, L., Barrette, B., Lovchike, J., Robinson, J.P., Hirleman, E.D. and Bhunia, A.K. 2014. Laser Optical Sensor, a Label-Free on-Plate Salmonella enterica Serovar Colony-Detection Tool. mBio 5(1): (DOI:10.1128/mBio.01019-13)
2. Koo, O.K., Amalaradjou, M.A.R., and Bhunia, A.K. 2012.Recombinant probiotic expressing Listeria adhesion protein attenuates Listeria monocytogenes virulence in vitro. PLoS One 7 (1): e29277
3. Huff, K., Aroonnual, A., Fleishman Littlejohn, A.E., Rajwa, B., Bae, E., Banada, P.P., Patsekin, V., Hirleman, E.D., Robinson, J.P., Richards, G.P., and Bhunia, A.K. 2012. Light-scattering sensor for real-time identification of Vibrio parahaemolyticus, V. vulnificus, and V. cholerae colonies on solid agar plate. Microbial Biotechnology, 5(5):607-620.
4. Jagadeesan, B., Fleishman Littlejohn, A.E., M.A.R. Amalaradjou, A.K. Singh, Mishra, K, La, D., Kihara, D., and Bhunia, A.K. 2011. N-terminal Gly224–Gly411 domain in Listeria Adhesion Protein interacts with host receptor Hsp60. PLoS One.6 (6):e20694.
5. Bi, L., Yang, L., Narsimhan, G., Bhunia, A.K., and Yao, Y. 2011. Carbohydrate nanoparticle mediated delivery for prolonged efficacy of antimicrobial peptide. J. Control. Release. 150: 150-156.
6. Burkholder, K.M. and Bhunia, A.K. 2010. Listeria monocytogenes uses LAP to promote bacterial transepithelial translocation, and induces expression of LAP receptor Hsp60. Infect. Immun. 78(12): 5062-5073.
7. Jagadeesan, B., Koo, O.K., Kim, K.P., Burkholder, K.M., Mishra, K.K., Aroonnual, A., and Bhunia, A.K. 2010. LAP, an alcohol acetaldehyde dehydrogenase enzyme in Listeria promotes bacterial adhesion to enterocyte-like Caco-2 cells only in pathogenic species. Microbiology-UK. 156 (9): 2782–2795.
8. Banerjee, P and Bhunia, A.K. 2010. Cell-based biosensor for rapid screening of pathogens and toxins. Biosens. Bioelectron. 26: 99–106.
9. Koo, O.K., Liu, Y., Shuaib, S., Bhattacharya, S., Ladisch, M.R., Bashir, R., and Bhunia, A.K. 2009. Targeted capture of pathogenic bacteria using mammalian cell receptor coupled with dielectrophoresis on biochip. Anal. Chem. 81:3094-3101.
10. Banada, P.P., Huff, K., Bae, E., Rajwa, B., Bayraktar, B., Aroonnual, A., Adil, A., Robinson, J.P., Hirleman, E.D., and Bhunia, A.K. 2009. Label - free detection of multiple bacterial pathogens using light scattering sensor. Biosens. Bioelectron. 24: 1685-1692.
11. Bhattacharya, S., Salamat, S., Morisette, D., Banada, P., Akin, D., Liu, Y.-S., Bhunia, A.K., Ladisch, M. and Bashir, R. 2008. PCR-based detection in a micro-fabricated platform. Lab Chip 8:1130-1136.
12. Nanduri, V., A.K. Bhunia, S.-I Tu, G.C. Paoli, and J.D. Brewster. 2007. SPR biosensor for the detection of L. monocytogenes using phage displayed antibody. Biosens.  Bioelectron. 23:248-252.
13. Akin, D., J. Sturgis, K. Ragheb, D. Sherman, K. Burkholder, J.P. Robinson, A.K. Bhunia, S. Mohammed, and R. Bashir. 2007. Bacteria-mediated delivery of nanoparticles into cells. Nat. Nanotechnol. 2:441-449.
14. Banada, P.P., S. Guo, B. Bayraktar, E. Bae, B. Rajwa, J. P. Robinson, E.D. Hirleman, and A.K. Bhunia. 2007. Optical forward scattering for detection of Listeria monocytogenes and other Listeria species. Biosens. Bioelectron. 22:1664-1671.
15. Kim, K.-P., B. Jagadeesan, K. Burkholder, Z.W. Jaradat, J.L. Wampler, A.A. Lathrop, M.T. Morgan, and A.K. Bhunia. 2006. Adhesion characteristics of Listeria adhesion protein (LAP) expressingEscherichia coli to Caco-2 cells and of recombinant LAP to eukaryotic receptor Hsp60 as examined in a surface plasmon resonance sensor. FEMS Microbiol. Lett. 256:324-332.
16. Maldonado, Y., J. Fiser, C. H. Nakatsu, and A.K. Bhunia. 2005. Cytotoxicity potential and genotypic characterization of Escherichia coli isolates from environmental and food sources. Appl. Environ. Microbiol. 71:1890-1898.
17. Gray, K. M., P. P. Banada, E. O’Neal, and A. K. Bhunia. 2005. Rapid Ped-2E9 cell-based cytotoxicity analysis and genotyping of Bacillus species. J. Clin. Microbiol. 43: 5865-5872.
18. Wampler, J.L., K-P. Kim, Z.W. Jaradat, and A.K. Bhunia. 2004. Heat-shock protein 60 acts as a receptor for the Listeria adhesion protein in Caco-2 cells. Infect. Immun. 72(2): 931-936.
19. Geng, T., M.T. Morgan, and A.K. Bhunia. 2004. Detection of low levels of Listeria monocytogenes cells by using a fiber-optic immunosensor. Appl. Environ. Microbiol. 70:6138-6146.
20. Jaradat, J.W. and A.K. Bhunia. 2003. Adhesion, Invasion, and translocation characteristics of Listeria monocytogenes serotypes in a Caco-2 cell and mouse model. Appl. Environ. Microbiol. 69 (6): 3640-3645.

 

 

 

 

 

 

 

 

 

 

 

 
 
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