Publications

 

  For reprints please contact Dr. Bhunia (bhunia@purdue.edu)

Publications are listed in chronological order.

Clickable (gold) links wll direct to respecitve article or abstract.

 

Refereed Publications


Pathogen Detection and Inactivation

 

1.     Bhunia, A.K., M.C. Johnson, and B. Ray. 1987. Direct detection of antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulfate polyacrylamide gel electrophoresis. J. Indust. Microbiol. 2: 319-322.

2.     Bhunia, A.K., M.C. Johnson, and B. Ray. 1988. Purification, characterization, and antimicrobial spectrum of a bacteriocin produced by Pediococcus acidilactici. J. Appl. Bacteriol. 65: 261-268.

3.     Bhunia, A.K., M.C. Johnson, B. Ray, and L.E. Belden. 1990.  Antigenic property of pediocin AcH, produced by Pediococcus acidilactici H. J. Appl. Bacteriol. 69: 211-215.

4.     Bhunia, A.K., M.C. Johnson, B. Ray, and N. Kalchayanand. 1991. Mode of action of pediocin AcH from Pediococcus acidilactici H on sensitive bacterial strains. J. Appl. Bacteriol. 70: 25-33.

5.     Bhunia, A.K., P.H. Ball, A.T. Faud, B.W. Kurz, J. Emerson, and M.G Johnson. 1991. Development and characterization of a monoclonal antibody specific for Listeria monocytogenes and L. innocua. Infect. Immun. 59: 3176-3184.

6.     Motlagh, A.M., A.K. Bhunia, M.G. Johnson, T.A. Hansen, F. Szostek, and B. Ray. 1992. Nucleotide sequence of pap-gene (pediocin AcH production) in Pediococcus acidilactici. Lett. Appl. Microbiol. 15: 45-48.

7.     Bhunia, A.K. and M.G. Johnson. 1992. A modified method to directly detect in SDS-PAGE the bacteriocin of Pediococcus acidilactici. Lett. Appl. Microbiol. 15: 5-7.

8.     Bhunia, A.K. and M.G. Johnson. 1992. Monoclonal antibody-colony immunoblot method specific for isolation of Pediococcus acidilactici from foods and correlation with pediocin (bacteriocin) production. Appl. Environ. Microbiol. 58: 2315-2320.

9.     Bhunia, A.K., P.H. Ball, and M.G. Johnson. 1992. A 20-24 h microcolony-immunoblot technique to directly detect and enumerate Listeria monocytogenes inoculated into foods. J. Rapid Methods Automat. Microbiol. 1: 67-82.

10. Bhunia, A.K., and M.G. Johnson. 1992.  Monoclonal antibody specific for Listeria monocytogenes associated with 66-kDa cell surface antigen. Appl. Environ. Microbiol. 58: 1924-1929.

11.  Bhunia, A.K., T.K. Bhowmik, and M.G. Johnson. 1994. Determination of bacteriocin encoding plasmids of Pediococcus acidilactici strains by Southern hybridization. Lett. Appl. Microbiol. 18: 168-170.

12.  Bhunia, A.K. 1994. Monoclonal antibody based enzyme immunoassay for pediocin of Pediococcus acidilactici. Appl. Environ. Microbiol. 60: 2692-2696.

13.  Bhunia, A.K., P.J. Steele*, D.G. Westbrook, L.A. Bly, T.P. Maloney and M.G. Johnson. 1994. A six-hour in vitro virulence assay for Listeria monocytogenes using myeloma and hybridoma cells from murine and human sources. Microb. Pathog. 16: 99-110.

14.  Bhunia, A.K., D.G. Westbrook*, R. Story, and M.G. Johnson. 1995. Frozen stored murine hybridoma cells can be used to determine the virulence of Listeria monocytogenes. J. Clin. Microbiol. 33: 3349-3351.

15.  Goff*, J., A.K. Bhunia, and M.G. Johnson. 1996. Complete inhibition of low levels of Listeria monocytogenes on refrigerated chicken meat with pediocin AcH bound to heat killed Pediococcus acidilactici cells. J. Food Protect. 59 (11): 1187-1192.

16.  Bhunia, A.K. 1997. Antibodies to Listeria monocytogenes. Crit. Rev. Microbiol. 23 (2): 77-107.

17.  Nannapaneni, R., R. Story, A.K. Bhunia, and M.G. Johnson. 1998. Unstable expression and thermal instability of species-specific cell surface epitope associated with 66-kilodalton antigen recognized by monoclonal antibody EM-7G1 within serotypes of Listeria monocytogenes grown in nonselective and selective broths. Appl. Environ. Microbiol. 64: 3070-3074.

18.  Nannapaneni, R., R. Story, A.K. Bhunia, and M.G. Johnson. 1998. Reactivities of genus-specific monoclonal antibody EM-6E11 against Listeria species and serotypes of Listeria monocytogenes grown in nonselective and selective enrichment broth media. J. Food Protect. 61: 1195-1198.​

19. Bhunia, A.K. and D.G. Westbrook. 1998. Alkaline phosphatase release assay to determine cytotoxicity for Listeria species. Lett. Appl. Microbiol. 26: 305-310.

20. Amoril, J.G. and A.K. Bhunia. 1999. Immunological and cytopathogenic properties of Listeria monocytogenes isolated from naturally contaminated raw meats. J. Food Safety 19: 195-207.

21.  Westbrook, D.G. and A.K. Bhunia. 2000. Dithiothreitol enhances Listeria monocytogenes mediated cell cytotoxicity. Microbiol. Immunol. 44: 431-438.

22.  Roberts, P.H., K.C. Davis*, W.R. Garstka, and A.K. Bhunia. 2001. Lactate dehydrogenase release assay from vero cells to distinguish verotoxin producing Escherichia coli from non-verotoxin producing strains. J. Microbiol. Methods 43: 171-181.

23.  Gomez, R., R. Bashir, A. Sarikaya, M.R. Ladisch, J. Sturgis, J.P. Robinson, T. Geng, A.K. Bhunia, H.L. Apple and S.T. Wereley. 2001. Microfluidic biochip for impedance spectroscopy of biological species. Biomed. Microdev. 3: 201-209.

24.  Singh*, N., R.K. Singh, A.K. Bhunia and R.L. Stroshine. 2002. Different surface inoculation methods with Escherichia coli O157:H7 affect the effectiveness of sanitizers on shredded lettuce. Food Microbiol. 19:183-193.

25. Jaradat, Z., G. E. Schutze, and A.K. Bhunia. 2002. Genetic homogeneity among Listeria monocytogenes strains from infected patients and meat products from two geographic locations determined by phenotyping, ribotyping and PCR analysis of virulence genes. Int. J. Food Microbiol. 76:1-10.

26.  Gomez, R., R. Bashir, and A.K. Bhunia. 2002. Microscale electronic detection of bacterial metabolism. Sens. Actuat. B: Chem. 86: 198-208.

27.  Chang, H., A. Ikram, M. Young, R. Bashir, F. Kosari, G. Vasmatzis and A. Bhunia. 2002. Electrical characterization of micro-organisms using microfabricated devices. J. Vacuum Sci. Technol. B. 20(5): 2058-2064.

28. Singh, N., R.K. Singh, A.K. Bhunia, and R.L. Stroshine. 2002. Efficacy of chlorine dioxide, ozone, and thyme essential oil, or a sequential washing in killing Escherichia coli O157:H7 on lettuce and baby carrots. LWT-Food Sci. Technol. 35: 720-729.

29.  Singh, N., R.K. Singh, and A.K. Bhunia. 2003. Sequential disinfection of Escherichia coli O157:H7 inoculated alfalfa seeds before and during sprouting using aqueous chlorine dioxide, ozonated water and thyme essential oil. LWT-Food Sci. Technol. 36: 235-243.

30.  Singh*, A., R.K. Singh, A.K. Bhunia, N. Singh. 2003. Efficacy of plant essential oils as antimicrobial agents against Listeria monocytogenes in hotdogs. LWT-Food Sci. Technol.  36: 787-794.

31. Huang, T., J. Sturgis, R. Gomez, T. Geng, R. Bashir, A.K. Bhunia, J.P. Robinson, and M.R. Ladisch. 2003. Composite surface for blocking bacterial adsorption on protein biochips. Biotechnol. Bioeng. 81:618-624.

32.  Hahm, B.-K., Y. Maldonado, A.K. Bhunia, and C.H. Nakatsu. 2003. Subtyping of foodborne and environmental isolates of Escherichia coli by multiplex PCR, rep-PCR, PFGE, ribotyping and AFLP. J. Microbiol. Methods 53: 387-399

33. Huang*, T., T. Geng, D. Akin, W.J. Chang, J. Sturgis, R. Bashir, A.K. Bhunia, J.P. Robinson, and M.R. Ladisch. 2003. Micro-assembly of functionalized particulate monolayers on C18-derivatized SiO2 surfaces. Biotechnol. Bioeng. 83: 416-427.

34.  Davis, K.C., C.H. Nakatsu, R. Turco, S. Weagent, and A.K. Bhunia. 2003. Analysis of environmental Escherichia coli isolates for virulence genes using the TaqMan system. J. Appl. Microbiol. 95: 612-620.

35.  Shroyer, M. and A.K. Bhunia. 2003. Development of a rapid 1-h fluorescence-based cytotoxicity assay for Listeria species. J. Microbiol. Methods. 55: 35-40.

36.  Geng, T., K-.P. Kim, D. Sherman, R. Gomez*, R. Bashir, M. Ladisch, and A.K. Bhunia. 2003. Expression of cellular antigens of Listeria monocytogenes that react with monoclonal antibodies C11E9 and EM-7G1 under acid-, salt- or temperature-induced stress environments. J. Appl. Microbiol. 95:762-772.

37.  Lathrop, A.L., Z.W. Jaradat, T. Haley, and A.K. Bhunia. 2003. Characterization and application of a Listeria monocytogenes reactive monoclonal antibody C11E9 in a resonant mirror biosensor. J. Immunol. Methods. 281: 119-128.

38.  Jaradat, Z.W., J.H. Bzikot, J. Zawistowski, and A.K. Bhunia. 2004. Optimization of a rapid dot-blot immunoassay for detection of Salmonella enterica serovar Enteritidis in poultry products. Food Microbiol. 21: 761-769.

39.  Ariefdjohan, M.W., P.E. Nelson, R.K. Singh, A.K. Bhunia, V.M. Balasubramaniam, and N. Singh. 2004. Efficacy of high hydrostatic pressure treatment in reducing Escherichia coli O157 and Listeria monocytogenes in alfalfa seeds. J. Food Sci. 69 (5): M117-120.

40.  Geng, T., M.T. Morgan, and A.K. Bhunia. 2004. Detection of low levels of Listeria monocytogenes cells by using a fiber-optic immunosensor. Appl. Environ. Microbiol. 70: 6138-6146.

41.  Gray, K.M., and A.K. Bhunia. 2005. Specific detection of cytopathogenic Listeria monocytogenes using a two-step method of immunoseparation and cytotoxicity analysis. J. Microbiol. Methods 60:259-268.

42.  Chen*, W.-T., R.L. Hendrickson, C.-P. Huang*, D. Sherman, T. Geng, A.K. Bhunia, and M.R. Ladisch. 2005. Mechanistic study of membrane concentration and recovery of Listeria monocytogenes. Biotechnol. Bioeng. 89:261-273.

43.  Maldonado*, Y., J. Fiser, C. H. Nakatsu, and A.K. Bhunia. 2005. Cytotoxicity potential and genotypic characterization of Escherichia coli isolates from environmental and food sources. Appl. Environ. Microbiol. 71 (4): 1890-1898.

44.  Chen*, W-T., T. Geng, A.K. Bhunia, and M.R. Ladisch. 2005. Membrane for selective capture of microbial pathogen Listeria monocytogenes. AIChE Journal. 51 (12):3305-3308.

45.  Gray*, K. M., P. P. Banada, E. O’Neal, and A. K. Bhunia. 2005. Rapid Ped-2E9 cell-based cytotoxicity analysis and genotyping of Bacillus species. J. Clin. Microbiol. 43 (12): 5865-5872.

46.  Yang, L., P.P. Banada, Y.S. Liu, A.K. Bhunia, and R. Bashir. 2005. Conductivity and pH dual detection of growth profile of healthy and stressed Listeria monocytogenes. Biotechnol. Bioeng. 92(6):685-693.

47.  Zhao, J., S. Jedlicka, J. Lannu, A.K. Bhunia, and J.L. Rickus. 2006. Liposome-doped nanocomposites as artificial-cell-based biosensors: Detection of listeriolysin O. Biotechnol. Prog. 22: 32-37.

48.  Hahm, B. K and A.K. Bhunia. 2006. Effect of environmental stresses on antibody-based detection of Escherichia coli O157:H7, Salmonella enterica serotype Enteritidis and Listeria monocytogenes. J. Appl. Microbiol. 100: 1017-1027.

49.  Banada, P.P., Y.S. Liu, L. Yang, R. Bashir, and A.K. Bhunia. 2006. Performance evaluation of a low conductive growth medium (LCGM) for growth of healthy and stressed Listeria monocytogenes and other common bacterial species. Int. J. Food Microbiol. 111:12-20.

50.  Wang*, H. Y., A.K. Bhunia, and C. Lu. 2006. A microfluidic flow-through device for high throughput electrical lysis of bacterial cells based on continuous DC voltage. Biosens. Bioelectron. 22: 582-588.

51. Lathrop, A., K. Huff, and A.K. Bhunia. 2006. Prevalence of antibodies reactive to pathogenic and nonpathogenic bacteria in preimmune serum of New Zealand white rabbits. J. Immunoass. Immunochem. 27:351-361.

52.  Bayraktar, B., P.P. Banada, E. D. Hirleman, A.K. Bhunia, J. Paul Robinson, and B. Rajwa. 2006. Feature extraction from light-scatter patterns of Listeria colonies for identification and classification. J. Biomed. Opt. 11(3) 034006-1-034006-8.

53. Geng, T., B.K. Hahm, and A.K. Bhunia. 2006. Selective enrichment media affect the antibody-based detection of stress-exposed Listeria monocytogenes due to differential expression of antibody-reactive antigens identified by protein sequencing. J. Food Protect. 69(8):1879-1886.

54.  Yang, L., P.P. Banada, R. Chatni, K.S. Lim, A.K. Bhunia, M. Ladisch, and R. Bashir. 2006. A multifunctional micro-fluidic system for dielectrophoretic concentration coupled with immuno-capture of low number of Listeria monocytogenes. Lab Chip 6:896-905.

55. Geng, T., J. Uknalis, S.-I. Tu, and A.K. Bhunia. 2006. Fiber-optic biosensor employing Alexa-Fluor conjugated antibody for detection of Escherichia coli O157:H7 from ground beef in four hours. Sensors 6:796-807.

56.  Nanduri, V., G. Kim, M.T. Morgan, D. Ess, B.-K. Hahm, A. Kothapalli, A. Valadez, T. Geng, A.K. Bhunia. 2006. Antibody immobilization on waveguides using a flow–through system show improved Listeria monocytogenes detection in an automated fiber optic biosensor: RAPTOR. Sensors 6: 808-822.

57.  Gehring, A.G., D.M. Albin, A.K. Bhunia, S.A. Reed, S.-I Tu, and J. Uknalis. 2006. Antibody microarray detection of Escherichia coli O157:H7:  Quantification, assay limitations, and capture efficiency. Anal. Chem. 78:6601-6607.

58.  Morgan, M.T., G. Kim, D.R. Ess, A. Kothapalli, B.K. Hahm, and A.K. Bhunia.  2006. Binding inhibition assay using fiber-optic based biosensor for the detection of foodborne pathogens. Key Eng. Materials 321-323:1145-1150.

59.  Kim, G., Morgan, M.T., D.R. Ess, B.K. Hahm, A. Kothapalli, A. Valadez, and A.K. Bhunia.  2006. Detection of Listeria monocytogenes using an automated fiber-optic biosensor: Raptor. Key Eng. Materials 321-323:1168-1171.

60.  Banada, P.P., S. Guo, B. Bayraktar, E. Bae, B. Rajwa, J. Paul Robinson, E.D. Hirleman, and A.K. Bhunia. 2007. Optical forward scattering for detection of Listeria monocytogenes and other Listeria species. Biosens. Bioelectron. 22:1664-1671.

61.  Banerjee, P.,  M.T. Morgan, J.L. Rickus, K. Ragheb, C. Corvalan, J. P. Robinson, and A.K. Bhunia. 2007. Hybridoma Ped-2E9 cells cultured under modified conditions can sensitively detect Listeria monocytogenes and Bacillus cereus. Appl. Microbiol. Biotechnol. 73:1423-1434.

62.  Bae*, E., Banada, P.P., Huff, K., Bhunia, A.K., Robinson, J.P., and Hirleman, E.D. 2007. Biophysical modeling of forward scattering from bacterial colonies using scalar diffraction theory. Appl. Opt. 46: 3639-3648.

63.  Bhunia, A.K., Banada, P.P., Banerjee, P., Valadez, A., and Hirleman, E.D. 2007. Light scattering, fiber optic and cell-based sensor for sensitive detection of foodborne pathogens. J. Rapid Methods Automat. Microbiol. 15: 121-145.

64.  Kim, G., Morgan, M.T., D.R. Ess, B.K. Hahm, A. Kothapalli, and A.K. Bhunia.  2007. An automated fiber-optic biosensor based binding inhibition assay for the detection of Listeria monocytogenes. Food Sci. Biotechnol. 16(3): 337-342.

65.  Nanduri, V., A.K. Bhunia, S.-I Tu, G.C. Paoli, and J.D. Brewster. 2007. SPR biosensor for the detection of L. monocytogenes using phage displayed antibody. Biosens. Bioelectron. 23:248-252.

66.  Bhunia, A.K. 2008. Biosensors and bio-based methods for the separation and detection of foodborne pathogens. Adv. Food Nutr. Res. 54: 1-44.

67. Lathrop, A.L., P.P. Banada, and A.K. Bhunia. 2008. Differential expression of InlB and ActA in Listeria monocytogenes in selective and nonselective enrichment broths. J. Appl. Microbiol. 104:627-639.

68.  Banerjee, P., Lenz, D., Robinson, J.P, Rickus, J.L., and Bhunia, A.K. 2008. A novel and simple cell-based detection system with collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins. Lab. Invest. 88:196-206.

69.  Bae, E., Banada, P.P., Huff, K., Bhunia, A.K., Robinson, J.P., and Hirleman, E.D. 2008. Analysis of time-resolved scattering from macroscale bacterial colonies. J. Biomed. Opt. 13(1): 014010.

70.  Bao, N., Jagadeesan, B., Bhunia, A.K., Yao, Y., and Lu, C. 2008. Quantificaiton of bacterial cells based on autofluorescence on a microfluidic platform. J. Chromat. A 1181:153-158.

71. Ngamwongsatit, P., Banada, P.P., Panbangred, W., and Bhunia, A.K. 2008. WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line. J. Microbiol. Methods 73: 211-215.

72. Liu, Y.-S., Banada, P.P., Bhattacharya, S., Bhunia, A.K. and Bashir, R. 2008. Electrical characterization of DNA molecules in solution using impedance measurements. Appl. Phys. Lett. 92:143902.

73.  Yang, L., Banada, P.P., Bhunia, A.K., Bashir, R. 2008. Effects of dielectrophoresis on growth, viability, and immuno-reactivity of Listeria monocytogenes. J. Biol. Eng. 2:6.

74.  Kim, H. and Bhunia, A.K. 2008. SEL, a selective enrichment broth for simultaneous growth of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes. Appl. Environ. Microbiol. 74 (15): 4853-4866.

75.  Bhattacharya, S., Salamat, S., Morisette, D., Banada, P., Akin, D., Liu, Y.-S., Bhunia, A.K., Ladisch, M. and Bashir, R. 2008. PCR-based detection in a micro-fabricated platform. Lab Chip 8:1130-1136.

76.  Kim, G., Morgan, M., Hahm, B.K., Bhunia, A., Mun, J.H. and Om, A.S. 2008. Interdigitated microelectrode based impedance biosensor for detection of Salmonella Enteritidis in food samples. J. Phys.: Conf. Series 100:052044.​

77. Bae, E., Aroonnual, A., Bhunia, A.K., Robinson, J.P., and Hirleman, E.D. 2009. System automation for bacterial colony detection and identification instrument via forward-scattering. Meas. Sci. Technol. 20:015802.

78.  Banada, P.P., Huff, K., Bae, E., Rajwa, B., Bayraktar, B., Aroonnual, A., Adil, A., Robinson, J.P., Hirleman, E.D., and Bhunia, A.K. 2009. Label-free detection of multiple bacterial pathogens using light scattering sensor. Biosens. Bioelectron. 24: 1685-1692.

79.  Banerjee, P., and Bhunia. A.K. 2009. Mammalian cell-based biosensors for pathogens and toxins. Trends Biotechnol. 27 (3): 179-188.

80.  Kim, G., Moon, J.H., Hahm, B.K., Morgan, M., Bhunia, A., and Om, A.S. 2009. Rapid detection of Salmonella enteritidis in pork samples with impedimetric biosensor: Effect of electrode spacing on sensitivity. Food Sci. Biotechnol. 18: 89-94.

81.  Koo, O.K., Liu, Y., Shuaib*, S., Bhattacharya, S., Ladisch, M.R., Bashir, R., and Bhunia, A.K. 2009. Targeted capture of pathogenic bacteria using mammalian cell receptor coupled with dielectrophoresis on biochip. Anal. Chem. 81:3094-3101.​

82.  Valadez, A.M., Lana, C.A., Tu, S.I., Morgan, M.T., and Bhunia, A.K. 2009. Evanescent wave fiber-optic biosensor for detection of Salmonella from food. Sensors. 9: 5810-5824.

83.  Bueno, V.F.F., Banerjee, P., Banada, P.P., Mesquita, A.J., Marques, E.G.L., and Bhunia, A.K. 2010. Characterization of Listeria monocytogenes isolates of food and human origins from Brazil using molecular typing procedures and in vitro cell culture assays. Int. J. Environ. Health Res. 20(1):43-59.

84.  Ohk, S.H., Koo, O.K., Sen, T., Yamamoto, C.M., and Bhunia, A.K. 2010. Antibody-aptamer functionalized fiber-optic biosensor for specific detection of Listeria monocytogenes from food. J. Appl. Microbiol. 109:808-817.

85.  Akova, F., Dundar, M., Jo Davisson, V., Hirleman, E.D., Bhunia, A.K., Robinson, J.P., and Rajwa, B. 2010.  A machine-learning approach to detecting unknown bacterial serovars. Statistical Analysis and Data Mining 3(5):289-301.

86.  Banerjee, P. and Bhunia, A.K. 2010. Cell-based biosensor for rapid screening of pathogens and toxins. Biosens. Bioelectron. 26: 99–106.

87.  Jagadeesan, B., Koo*, O.K., Kim*, K.-P., Burkholder, K.M., Mishra, K.K., Aroonnual, A., and Bhunia, A.K. 2010. LAP, an alcohol acetaldehyde dehydrogenase enzyme in Listeria promotes bacterial adhesion to enterocyte-like Caco-2 cells only in pathogenic species. Microbiology-UK. 156 (9): 2782–2795.

88.  Bae, E., Bai, N., Bhunia, A.K., Robinson, J.P. and Hirleman, E.D. 2010. Modeling light propagation through bacterial colonies and its correlation with forward scattering patterns. J. Biomed. Opt. 15(4): 019004.

89.  Rajwa, B., Dundar, M.M., Akova, F., Bettasso, A., Patsekin, V., Hirleman, E.D, Bhunia, A.K., Robinson, J.P. 2010. Discovering unknown: detection of emerging pathogens using label-free light scattering system, Cytometry Part A, 77A: 1103-1112.

90.  Banerjee, P., Franz, B, and Bhunia. A.K. 2010. Mammalian cell-based sensor system. Adv. Biochem. Eng. Biotechnol. 117: 21-55.

91.  Bhunia, A.K. 2010. Mammalian cell-based sensors: a rapid screening tool for pathogens and toxins in food. Food Engineering and Ingredients. Oct issue: 15-17. (invited)

92.  Bae, E., Bai, N., Aroonnual, A., Bhunia, A.K., and Hirleman, E.D., 2011. Label-free identification of bacterial microcolonies via elastic scattering. Biotechnol. Bioeng. 108(3):637-644.

93.  Sirsat, S.A., Burkholder, K.M., Dowd, S.E., Muthaiyan, A., Bhunia, A.K., and Ricke, S.C. 2011. Effect of sublethal heat stress on S. Typhimurium virulence. J. Appl. Microbiol. 110: 813-822.

94.  Bae, E., Aroonnual, A., Bhunia, A.K., and Hirleman, E.D. 2011. On the sensitivity of forward scattering patterns from bacterial colonies to media composition. J. Biophoton. 4:236-243.

95.  Bi, L., Yang, L., Narsimhan, G., Bhunia, A.K., and Yao, Y. 2011. Designing carbohydrate nanoparticle for prolonged efficacy of antimicrobial peptide. J. Control. Release 150: 150-156.

96.  Bi, L., Yang, L., Bhunia, A.K., and Yao, Y. 2011. Carbohydrate nanoparticle-mediated colloidal assembly for prolonged efficacy of bacteriocin against food pathogen. Biotechnol. Bioeng. 108 (7): 1529-1536.

97.  Bhunia, A.K. 2011. Rapid pathogen screening tools for food safety. Food Technol. 65(2):38-43

98.   Koo, O.K., Aroonnual, A., and Bhunia, A.K. 2011. Human heat shock protein 60 receptor coated paramagnetic beads show improved capture of Listeria monocytogenes in presence of other Listeria in food. J. Appl. Microbiol. 111: 93-104.

99.  Jaradat, Z.W., Rashdan, A.M., Ababneh, Q.O., Jaradat, S.A., and Bhunia, A.K. 2011. Characterization of surface proteins of Cronobacter muytjensii using monoclonal antibodies and MALDI-TOF Mass spectrometry. BMC Microbiol. 11:148.

100.        Salm, E., D. Marchwiany, D., Liu, Y.S., Morisette, D., He, Y., Bhunia, A., and Bashir, R. 2011. Electrical detection of dsDNA and polymerase chain reaction amplification. Biomed. Microdev.  13 (6): 973-982.

101.        Robinson, J.P., Rajwa, B.P., Bae, E., Patsekin, V., Roumani, A.M., Bhunia, A.K., Dietz, J.E., Davidson, V.J., Dunder, M.M., Thomas, J., Hirleman, E.D. 2011. Using scattering to identify bacterial pathogens.  Opt. Photon. News. 22(10): 21-27.

102.        Mishra, K.K., Mendonca, M., Aroonnual, A., Burkholder, K.M. and Bhunia, A.K. 2011. Genetic organization and molecular characterization of secA2 locus in Listeria species. Gene 489:76-85.

103.        Gehring, A.G., Albin, D.M., Bhunia, A.K., Kim, H., Reed, S.A., and Tu, S-I. 2012. Mixed culture enrichment of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica, and Yersinia enterocolitica, Food Control 26:269-273.

104. Bae, E., Patsekin, V., Rajwa, B., Bhunia, A.K., Hirleman, E.D., Holdman, C., and Robinson, J.P.   2012. Development of a microbial high-throughput screening instrument based on elastic light scatter patterns. Rev. Scientific Instruments, 83:044304.

105.        Huff, K., Aroonnual, A., Fleishman Littlejohn, A.E., Rajwa, B., Bae, E., Banada, P.P., Patsekin, V., Hirleman, E.D., Robinson, J.P., Richards, G.P., and Bhunia, A.K. 2012. Light-scattering sensor for real-time identification of Vibrio parahaemolyticus, V. vulnificus, and V. cholerae colonies on solid agar plate. Microb. Biotechnol. 5(5):607-620.

106.        Bae, E., Kim, H., McNally, H.A., Bhunia, A.K. and Hirleman, E.D., 2012. Investigation of the presence of rod-shaped bacteria on food surface via elastic light scattering. Adv. Biosci. Biotechnol. 3:344-352.

107.        Mialon, M., Tang, Y., Singh, A.K., Bae, E., and Bhunia, A.K.  2012. Effects of preparation and storage of agar media on the sensitivity of bacterial forward scattering patterns. Open J. Appl. Biosens. 1:26-35.

108.        Mendonça, M., Conrad, N.L., Conceição, F.R., Moreira, A.N., da Silva, W.P., Aleixo, J.A.G., and Bhunia, A.K. 2012. Highly specific fiber optic immunosensor coupled with immunomagnetic separation for detection of low levels of Listeria monocytogenes and L. ivanovii. BMC Microbiol. 12:275.

109.        Ohk, S-H., and Bhunia, A.K. 2013. Multiplex fiber optic biosensor for detection of Listeria monocytogenes, Escherichia coli O157:H7 and Salmonella enterica from ready-to-eat meat samples. Food Microbiol.33:166-171.

110.        Ahmed, W.M., Bayraktar, B., Bhunia, A.K., Hirleman, E.D., Robinson, J.P., and Rajwa, B. 2013. Classification of bacterial contamination using image processing and distributed computing. IEEE J. Biomed. Health Informatics. 17:232-239.

111. Kim, H., Bai, N., Bhunia, A.K., King, G.B., Hirleman, E.D., and Bae, E. 2013. Development of an integrated optical analyzer for characterization of growth dynamics of bacterial colonies. J. Biophoton. 6:929-937.

112.        Li, X., Ximenes, E., Amalaradjou, M.A.R., Vibbert, H.B., Foster, K., Jones, J., Liu, X., Bhunia, A.K. and Ladisch, M.R. 2013. Rapid Sample Processing for Foodborne Pathogen Detection via Crossflow Microfiltration. Appl. Environ. Microbiol. 79:7048-7054.

113.        Singh, A.K., Bettasso, A.M., Bae, E., Rajwa, B., Dundar, M.M., Forstere, M.D., Liu, L., Barrette, B., Lovchike, J., Robinson, J.P., Hirleman, E.D. and Bhunia, A.K. 2014. Laser optical sensor, a label-free on-plate Salmonella enterica serovar colony-detection tool. mBio 5(1):e01019-13.

114.  Cho, I-H., Radadia, A., Farrokhzad, K., Ximenes, E., Bae, E., Singh, A.K., Oliver, H., Ladisch, M.R., Bhunia, A.K., Applegate, B., Mauer, L., Bashir, R., Irudayaraj, J. 2014. Nano/Micro and spectroscopic approaches to food pathogen detection. Annu. Rev. Anal. Chem. 7:65-88.

115.        Lathrop, A.A., Bailey, T.W., Kim, K.P., and Bhunia, A.K. 2014. Pathogen-specific antigen target for production of antibodies produced by comparative genomics, Antibody Technol. J. 4:4 (1-10).

116. Bhunia, A.K. 2014. One day to one hour: how quickly can foodborne pathogens be detected? Future Microbiol. 9(8):935-946. (Invited)

117.  Tang, Y., Kim, H., Singh, A.K., Aronnual, A., Bae, E., Rajwa, R., Fratamico, P.M., and Bhunia, A.K. 2014. Light scattering sensor for direct identification of colonies of Escherichia coli serogroups O26, O45, O103, O111, O121, O145 and O157, PLoS ONE. 9(8): e105272.

118.        Kim, H., Singh, A.K., Bhunia, A.K., and Bae, E., 2014. Laser-induced speckle scatter patterns in Bacillus colonies. Front. Microbiol. 5: Article No. 537 (1-9).

119. He, Y., Reed, S., Bhunia, A.K., Gehring, A., Nguyen, L.-H., and Irwin, P.L. 2015. Rapid identification and classification of Campylobacter spp. using laser optical scattering technology, Food Microbiol. 47:28-35.

120.        Singh, A.K., Sun, X., Bai, X., Kim, H., Abdalhaseib, M., Bae, E., and Bhunia, A.K. 2015. Label-free, non-invasive light scattering sensor for screening of Bacillus colonies. J. Microbiol. Methods 109:56-66.

121.        Josefsen, M. H., Bhunia, A.K., Olsson Engvall, E., Søndergaard, M. S. R. and Hoorfar, J. 2015. Monitoring Campylobacter in the poultry production chain–From culture to genes and beyond. J. Microbiol. Methods 112:118-125.

122. Kim, H., Doh, I-J., Bhunia, A.K., King, G.B., and Bae, E. 2015. Scalar diffraction modeling of multi-spectral forward scatter patterns from bacterial colonies. Optics Express 23 (7):8545-8554.

123.        Ansari, S., Bozkurt, F., Yazar, G., Ryan, V., Bhunia, A. and Kokini, J. 2015. Probing the distribution of gliadin proteins in dough and baked bread using conjugated quantum dots as a labeling tool. J. Cereal Sci. 63:41-48.

124. Il-Hoon Cho, I-H., Bhunia, A.K., Irudayaraj, J. 2015. Rapid pathogen detection by lateral-flow immunochromatographic assay with gold nanoparticle-assisted enzyme signal amplification. Int. J. Food Microbiol. 206: 60-66.

125.        Qiu, Y.L., He, Q-H., Xu, Y., Bhunia, A.K., Tu, Z., Chen, B., Liu, Y-Y. 2015. Deoxynivalenol-mimic nanobody isolated from a naïve phage display nanobody library and its application in immunoassay. Analytica Chimica Acta (In Press, 2015)

Pathogenesis

1.     Pandiripally, V.P., D.G. Westbrook, G.R. Sunki, and A.K. Bhunia. 1999. Surface protein p104 is involved in adhesion of Listeria monocytogenes to human intestinal cell line, Caco-2. J. Med. Microbiol. 48: 117-124.​

2.     Santiago, N.I., A. Zipf, and A.K. Bhunia. 1999. Influence of temperature and growth phase on expression of a 104-kDa Listeria adhesion protein in Listeria monocytogenes. Appl. Environ. Microbiol. 65: 2765-2769.

3.     Bhunia, A.K. and X. Feng. 1999. Examination of cytopathic effect and apoptosis in Listeria monocytogenes- infected hybridoma B-lymphocyte (Ped-2E9) lines. J. Microbiol. Biotechnol. 9: 398-403.

4.     Jaradat, Z.W. and A.K. Bhunia. 2002. Glucose and nutrient concentrations affect the expression of a 104-kDa Listeria adhesion protein in Listeria monocytogenes. Appl. Environ. Microbiol. 68 (10): 4876-4883.

5.     Menon, A., M. Shroyer, J. L. Wampler, C.B. Chawan, and A.K. Bhunia. 2003. In vitro study of Listeria monocytogenes infection to murine primary and human transformed B cells.  Comp. Immunol. Microbiol. Infect. Dis. 26 (3): 157-174.

6.    Jaradat, Z.W., J. L. Wampler, and A.K. Bhunia. 2003. A Listeria adhesion protein-deficient Listeria monocytogenes strain shows reduced adhesion primarily to intestinal cell lines. Med. Microbiol. Immunol. 192: 85-91.

7. Jaradat, J.W. and A.K. Bhunia. 2003. Adhesion, Invasion, and translocation characteristics of Listeria monocytogenes serotypes in a Caco-2 cell and mouse model. Appl. Environ. Microbiol. 69 (6): 3640-3645.

8.     Wampler, J.L., K-P. Kim, Z.W. Jaradat, and A.K. Bhunia. 2004. Heat-shock protein 60 acts as a receptor for the Listeria adhesion protein in Caco-2 cells. Infect. Immun. 72(2): 931-936.

9.     Zhang, P., J.L. Wampler, A.K. Bhunia, R.L. Whistler, K.M. Burkholder, and J.A. Patterson. 2004. Effects of arabinoxylans on activation of murine macrophages and growth performance of broiler chicks. Cereal Chem. 81 (4): 511-514.

10.  Kim, K.-P., B. Jagadeesan, K. Burkholder, Z.W. Jaradat, J.L. Wampler, A.A. Lathrop, M.T. Morgan, and A.K. Bhunia. 2006. Adhesion characteristics of Listeria adhesion protein (LAP) – expressing Escherichia coli to Caco-2 cells and of recombinant LAP to eukaryotic receptor Hsp60 as examined in a surface plasmon resonance sensor. FEMS Microbiol. Lett. 256:324-332.

11.   Kim, K.-P., B.K. Hahm, and A.K. Bhunia. 2007. The 2-cys peroxiredoxin deficient Listeria monocytogenes displays impaired growth and survival in the presence of hydrogen peroxide in vitro but not in mouse organs. Curr. Microbiol. 54:382-387.

12.  Akin, D., Sturgis, J., Ragheb, K., Sherman, D., Burkholder, K., Robinson, J.P., Bhunia, A.K., Mohammed, S. and Bashir, R. 2007. Bacteria-mediated delivery of nanoparticles into cells. Nat. Nanotechnol. 2:441-449.

13.  Banerjee, P., Merkel, G.J., and Bhunia, A.K. 2009. Lactobacillus delbrueckii ssp. bulgaricus B-30892 can inhibit cytotoxic effects and adhesion of pathogenic Clostridium difficile to Caco-2 cells. Gut Pathog. 1:8.

14.  Burkholder, K.M., Kim, K-P., Mishra, K., Medina, S., Hahm, B-K., Kim, H., and Bhunia, A.K. 2009. Expression of LAP, a SecA2-dependent secretory protein, is induced under anaerobic environment. Microbes Infect. 11:859-867.

15.  Burkholder, K.M. and Bhunia, A.K. 2009. Salmonella enterica serovar Typhimurium adhesion and cytotoxicity during epithelial cell stress is reduced by Lactobacillus rhamnosus GG. Gut Pathog. 1:14.

16.  Burkholder, K.M. and Bhunia, A.K. 2010. Listeria monocytogenes uses LAP to promote bacterial transepithelial translocation, and induces expression of LAP receptor Hsp60. Infect. Immun. 78(12): 5062-5073.

17.  Jagadeesan, B., Fleishman Littlejohn, A.E., Amalaradjou, M.A.R., Singh, A.K., Mishra, K., La, D., Kihara, D., and Bhunia, A.K. 2011. N-terminal Gly224–Gly411 domain in Listeria Adhesion Protein interacts with host receptor Hsp60. PLoS One.6 (6):e20694.

18.  Koo, O.K., Amalaradjou, M.A.R., and Bhunia, A.K. 2012.Recombinant probiotic expressing Listeria adhesion protein attenuates Listeria monocytogenes virulence in vitro. PLoS One 7 (1): e29277.

19.  Bhunia, A.K. 2012. Bioengineered Probiotics – A Solution to Broaden Probiotics Efficacy! (editorial) J. Nutr. Food Sci. 2:3: 1000e105

20.  Amalaradjou, M. A. R., and A. K. Bhunia. 2012. Modern approaches in probiotics research to control foodborne pathogens. Adv. Food Nutr. Res. 67:185-239. (Invited)

21.  Amalaradjou, M.A.R., and Bhunia, A.K. 2013. Bioengineered probiotics, a strategic approach to control enteric infections. Bioengineered 4 (6): 379-387 (Invited).

22.  Kim, H., and Bhunia, A.K. 2013. Secreted Listeria adhesion protein (Lap) influences Lap-mediated Listeria monocytogenes paracellular translocation through epithelial barrier. Gut Pathog. 5:16.

Text Books

 

1.     Bhunia, A.K. 2008. Microbial Foodborne Pathogens: Mechanisms and Pathogenesis. First Edition, Springer, New York (Published in English, and Russian)

2.     Ray, B., and Bhunia, A.K. 2008. Fundamental Food Microbiology, 4th Edition, CRC Press (Taylor and Francis group), Boca Raton, FL. (Published in English, Chinese, Spanish and Korean)

3.     Ray, B., and Bhunia, A.K. 2014. Fundamental Food Microbiology, 5th Edition, CRC Press (Taylor and Francis group), Boca Raton, FL.

Edited Books

 

1.     Foodborne Pathogens: Microbiology and Molecular Biology, 2005. Editors: P. M. Fratamico, A.K. Bhunia and J.L. Smith, Caister Academic Press, Ltd., Norfolk, UK.

 

1.     Advances in Homeland Security, 2006. Vol. 1, The Science of Homeland Security, Editors:  S. Amass, A. K.  Bhunia, A. Chaturvedi, D. Dolk, S. Peeta and M. Atallah, Purdue University Press, West Lafayette, IN

 

1.     High Throughput Screening for Food Safety Assessment: Biosensor Technologies, Hyperspectral Imaging and Practical Applications. 2015. Editors: A. K. Bhunia, Moon S. Kim, and C.R. Taitt. Woodhead Publishing, Cambridge, UK. Pages 523

 

Book Chapters

 

       Bhunia, A.K. and A. Lathrop. 2003. Foodborne Pathogen Detection, McGraw- Hill 2003 Year Book of Science and Technology, pp.320-323. McGraw-Hill Professional, New York, NY.

 

       Bhunia, A.K. and J. L. Wampler. 2005. Animal and cell culture models for foodborne bacterial pathogens. In Foodborne Pathogens: Microbiology and Molecular Biology, Editors: P. M. Fratamico, A.K. Bhunia, and J.L. Smith. Caister Academic Press, Ltd., Norfolk, UK, pp15-32.

 

       Paoli, G.C., A.K. Bhunia, and D.O. Bayles. 2005. Listeria monocytogenes. In Foodborne Pathogens: Microbiology and Molecular Biology, Editors: P. M. Fratamico, A.K. Bhunia and J.L. Smith, Caister Academic Press, Ltd., Norfolk, UK, pp 295-339.

 

       Bhunia, A.K. 2006. Detection of significant bacterial pathogens and toxins of interest in homeland security. In Advances in Homeland Security, Vol. 1, The Science of Homeland Security, Editors:  S. Amass, A. K.  Bhunia, A. Chaturvedi, D. Dolk, S. Peeta and M. Atallah, Purdue University Press, West Lafayette, IN pp 109-149.

 

       Geng, T., and A.K. Bhunia. 2007. Optical biosensors in foodborne pathogen detection. In Smart Biosensor Technology, Editors: G. K. Knopf and A.S. Bassi, CRC Press, Taylor and Francis group, Boca Raton, FL. Pp 505-519.

 

Wang, H.Y., P. P. Banada, A.K. Bhunia, and C. Lu. 2007. Rapid electrical lysis of bacterial cells in a microfluidic device. In Methods in Molecular Biology, Vol 385: Microchip-Based Assay Systems: Methods and Application. Editor: P. N. Floriano, Humana Press, Inc., Totowa, NJ. Pp 23-35.

 

       Banada, P.P. and Bhunia, A.K. 2008. Antibodies and immunoassays for detection of bacterial pathogens. Chapter 21, In Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems, Zourob, M.; Elwary, S; Turner, A (Eds.) Springer, New York, pp 567-602.

 

       Bhunia, A.K., Nanduri, V., Bae, E. and Hirleman, E.D., 2010. Biosensors, Foodborne Pathogen Detection. In Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology. Flickinger, M.C. (Ed), John Wiley & Sons, Inc., Hoboken, NJ.

 

       Goodridge, L.D., Fratamico, P., Christensen, B.B., Hoorfar, J., Griffiths, M., Carter, M., Bhunia, A.K., O’Kennedy, R. 2011. Strengths and shortcomings of advanced detection technologies. In Rapid Detection, Characterization and Enumeration of Foodborne Pathogens. Editor, J. Hoorfar. ASM Press, Washington DC. pp 15-45.

 

       Malorny, B., Bhunia, A.K., Aarts, H.J.M., Löfström, C., and Hoorfar, J. 2011. Salmonella in pork, beef, poultry and egg. In Rapid detection, characterization and enumeration of food-borne pathogens. Editor, J. Hoorfar, ASM Press, Washington DC. pp 179-194.

 

       Hoorfer, J., Christensen, B.B., Pagotto, F., Rudi, K., Bhunia, A.K., and Griffith, M. 2011. Future trends in rapid methods. In Rapid Detection, Characterization and Enumeration of Foodborne Pathogens. Editor, J. Hoorfar. ASM Press, Washington DC. pp 413-420.

 

       Bhunia, A.K., Bae, E., Rajwa, B., Robinson, J.P. and Hirleman, E.D. 2012. Utilization of optical forward scatter image biological database: Food-borne pathogen colony differentiation and detection. In Omics, Microbial Modeling, and Technologies in Foodborne Pathogens. Editors: Yan, X., Juneja, V., Fratamico, P., Smith, J.L., DEStech Publications, Inc. Lancaster, PA. Ch 19, pp 553-578.

 

       Bae, E. and Bhunia, A.K. 2013. Nano Optical Sensors for Food Safety and Security, In Optochemical Nanosensors, Editor, A. Cusano, F. J. Arregui, M. Giordano, and A. Cutolo. CRC Press, Taylor and Francis Group, Boca Raton, FL., Chapter 19, pp 497-512.

 

       Bhunia, A.K. 2013. Forthcoming New Technologies for Microbial Detection. In Guide to Foodborne Pathogens, Second Edition, R.G. Labbe and S. Garcia (ed), Wiley-Blackwell, Oxford, UK. Chapter 25; pp 414-421.

 

       Burkholder, K.M., and Bhunia, A.K. 2013. Listeria monocytogenes and Host Hsp60 - an Invasive Pairing. In Moonlighting Cell Stress Proteins in Microbial Infections, B. Henderson (Ed), Chapter 17, Springer, pp 267-282.

 

       Singh, A.K., Sarkar, P., Janaswamy, S., Yao, Y., and Bhunia, A.K. 2014. Encapsulation and Delivery of Antimicrobial Compounds, In Novel Food Preservation and Microbial Assessment Techniques, Chapter 8. Editor, Ioannis S. Boziaris, CRC Press, Boca Raton, FL. Pp. 218-236.

 

       Bhunia, A.K., Taitt, C.R., and Kim, M.S. 2015. High throughput screening strategies and technology platforms for detection of pathogens in food: An Introduction, In High Throughput Screening for Food Safety Assessment: Biosensor Technologies, Hyperspectral Imaging and Practical Applications. Chapter 1, Editors, A. K. Bhunia, M.S. Kim, and C.R. Taitt. Woodhead Publishing, Cambridge, UK. Pp. 1-9.

 

       Bae, E. and Bhunia, A.K. 2015. Label-free light scattering sensors for high throughput screening of microbes and toxins in food. In High Throughput Screening for Food Safety Assessment: Biosensor Technologies, Hyperspectral Imaging and Practical Applications. Chapter 6, Editors, A.K. Bhunia, M.S. Kim, and C.R. Taitt. Woodhead Publishing, Cambridge, UK. Pp. 149-162.

 

       Mendonca, M., and Bhunia, A.K. 2015. Fiber Optic Sensors for High Throughput Screening of Pathogens, In High Throughput Screening for Food Safety Assessment: Biosensor Technologies, Hyperspectral Imaging and Practical Applications. Chapter 14, Editors, A.K. Bhunia, M.S. Kim, and C.R. Taitt. Woodhead Publishing, Cambridge, UK. Pp. 249-259.

 

Monographs and Proceeding Articles

 

        Bhunia, A.K. 1986. Some characteristics of an antimicrobial substance produced by a strain of Pediococcus acidilactici used in meat fermentation. Animal Science Roundup Report, University of Wyoming. p. 41-44.

 

        Linton, R. and A.K. Bhunia. 1999. Listeria monocytogenes: Survival of the fittest. Purdue Extension Publication. FS-7: 1-2.

 

        Linton, R. and A.K. Bhunia. 1999. E. coli O157:H7 - Concerns and challenges for the next millennium. Purdue Extension Publication. FS-9: 1-3.

 

        Bhunia, A., Z.W. Jaradat, K. Naschansky, M. Shroyer, M. Morgan, R. Gomez, R. Bashir, and M. Ladisch. 2001. Impedance spectroscopy and biochip sensor for detection of Listeria monocytogenes. Proceedings of SPIE (Society for Photo Optical Instrumentation Engineers), 4206: 32-39.

 

        Singh, R.K., A. Bhunia, A. Singh. 2001. Light emission based biosensors for detection of food pathogens: a review. Proceedings of SPIE, 4206: 7-12.

 

        Singh, N., R.K. Singh, A. Bhunia, R.L. Stroshine, and J.E. Simon. 2001. Sequential disinfection of foodborne pathogens by ozone, chlorine dioxide, and natural plant extracts. Proceedings of SPIE, 4206: 159-166.

 

        Nebeker, B.M., B. Buckner, E. D. Hirleman, A. Lathrop, and A.K. Bhunia. 2001. Identification and characterization of bacteria on surfaces using polarized light scattering. Proceedings of SPIE, 4206: 224-234.

 

        Turco, R., S. Brouder, C. Nakatsu, A. Bhunia, J. Frankenberger, J. Harbor, G. Thomas. 2001. Watershed-scale assessments of E. coli contamination implications of source identification for public policy debate. EPA Proceedings, (2001 Water and Watersheds Progress Review), p17.

 

        Bashir, R., R. Gomez, H. Chang, H. Li, M. Ladisch and A.K. Bhunia. 2001. Applications of Micro-systems technology for characterization and detection of microorganisms. Proceeding of MEMS conference 2001, pages 11-13, August 24-26th, 2001, Berkeley, CA.

 

        Chang, H., A. Ikram, M. Young, R. Bashir, F. Kosari, G. Vasmatzis and A. Bhunia. 2001. A microfabricated device for the characterization of biological species. Mat. Res. Soc. Symp. Proc. (Proceedings of the Spring MRS 2001) 679E: B3.3.1 – B3.3.6, San Francisco, CA.

 

        Gomez, R., R. Bashir, A.K. Bhunia, and M.R. Ladisch. 2002. Microfabricated device for impedance-based detection of bacterial metabolism.  Mat. Res. Soc. Symp. Proc. (Proceedings of the Spring MRS 2002) 729: U4.6.1-U4.6.6. San Francisco, CA.

 

•      Bhunia, A.K., T. Geng, A. Lathrop, A. Valadez, and M. Morgan. 2004. Optical immunosensor for detection of Listeria monocytogenes and Salmonella Enteritidis from food. Proceedings of SPIE, 5271:1-6.

 

        Li, H., L. Yang, D. Akin, T. Geng, A. Bhunia, T.T. Huang, M. Ladisch, R. Bashir. 2005. Dielectrophoresis and antibody mediated selective capture of microorganism in micro-fluidic biochips. Transducers 05 – The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, Seoul, Korea, June 5-9, 2005.

 

       Banerjee, P., P. P. Banada, J.L. Rickus, M.T. Morgan, and A.K. Bhunia. 2005. A portable cell based optical detection device for rapid detection of Listeria and Bacillus toxins. Proceeding of SPIE, 5996:599602-1- 7.

 

        Wang, H.-Y., C. Lu, P.P. Banada, B. Jagadeesan, and A.K. Bhunia. 2005. Microfluidic pretreatment of bacterial cells for analysis of intracellular contents. Proceedings of SPIE 5996:599603-1- 10.

 

        Morgan, M.T., G. Kim, D.R. Ess, A. Kothapalli, and A.K. Bhunia.  2006. Binding inhibition assay using fiber-optic based biosensor for the detection of foodborne pathogens.  Proceedings of 1st International Conference on Advanced Nondestructive Evaluation.  Jeju, Korea.  Nov. 7 – 9, 2005.

 

        Bayraktar, B., Banada, P.P., Hirleman, E.D., Bhunia, A.K., Robinson, J.P., Rajwa. B. 2006. Bacterial phenotype identification using Zernike moment invariants. Proceedings of SPIE. 6080:155-162.

 

        Rajwa B., Bayraktar, B., Banada, P.P., Huff, K., Bae, E., Hirleman, E.D., Bhunia, A.K., Robinson, J.P. 2006. Noninvasive forward scattering system for rapid detection, characterization, and identification of Listeria colonies: Image processing and data analysis. Proceedings of SPIE. 6381:638105-1 - 638105-8.

 

       Bae, E., Banada, P.P., Bhunia, A.K., and Hirleman, E.D. 2006. Bio-physical modeling of time-resolved forward scattering by Listeria colonies. Proceedings of SPIE. 6381: 638107-1 – 638107-8.

 

        Tu, S-I., Geng, T., Uknalis, J., and Bhunia, A.K. 2006. Fiber-optic biosensor employing Alexa-Fluor conjugated antibodies for detection of Escherichia coli O157:H7 and Shiga-like toxins. Proceedings of SPIE 6381: 638106 (1 – 6).

 

        Bhunia, A.K. 2006. Control and prevention of Listeria monocytogenes in the dairy industry. 2006. Perspectivas E Avancos Da Qualidade Do Leite No Brasil. pp 259-260.

 

        Leavesley, S., Bayraktar, B., Venkatapathi, M., Hirleman, E.D., Bhunia, A.K., Robinson, J.P., Hassler, R., Smith, L., Rajwa, B. 2007. Quantification of morphology of bacterial colonies using laser scatter measurements and solid element optical modeling, Proceedings of SPIE 6446:64460E (1-6)

 

        Rajwa, B., Bayraktar, B., Banada, P.P., Huff, K., Bae, E., Hirleman, E.D., Bhunia, A.K., Robinson, J.P. 2007. Noninvasive forward-scattering system for rapid detection, characterization, and identification of bacterial colonies. Proceedings of SPIE 6554: 65540N-1 – 7.

 

        Wamiq M. Ahmed, W.M., Bayraktar, B., Bhunia, A.K., Hirleman, E.D., Robinson, J.P., and Rajwa, B. 2007. Rapid detection and classification of bacterial contamination using grid computing. IEEE Vol. 286-293.

 

•     Rajwa, B., Bayraktar, B., Banada, P.P., Huff, K., Bae, E., Hirleman, E.D., Bhunia, A.K., Robinson, J.P. 2008. Phenotypic analysis of bacterial colonies using laser light scatter and pattern-recognition techniques. Proceedings of SPIE 6864: 68640S (1–7).

 

        Bae, E., Lesmana, A., Bhunia, A.K., Robinson, J.P., and Hirleman, E.D. 2008. Development and optimization of two-dimensional centering algorithm for bacterial rapid detection system using forward scattering. Proceedings of SPIE 6849: 684905 (1-8).

 

        Bae, E., Bai, N., Aroonnual, A., Bhunia, A.K., Robinson, J.P., and Hirleman, E.D. 2009. Prediction of the light scattering patterns from bacteria colonies by a time-resolved Reaction-diffusion model and the scalar diffraction theory. Proceedings of SPIE. 7315: 73150A (1 – 7).

 

        Bai, N., Bae, E., Aroonnual, A., Bhunia, A.K., Robinson, J.P., and Hirleman, E.D. 2009. Development of a real-time system of monitoring the bacterial growth and registering the forward-scattering pattern. Proceedings of SPIE. 7315: 73150Z (1– 8).

 

        Rajwa, B., Dundar, M., Patsekin, V., Huff, K., Bhunia, A.K., Venkatapathi, M., Bae, E., Hirleman, E.D. and Robinson, J.P., 2009. Morphotypic analysis and classification of bacteria and bacterial colonies using laser light-scattering, pattern recognition, and machine-learning system. Proceedings of SPIE, 7306.

 

        Dundar, M. M, Hirleman, E.D., Bhunia, A.K., Robinson, J.P., and Rajwa, B. 2009. Learning with a non-exhaustive training dataset: a case study: detection of bacteria cultures using optical-scattering technology. In Proceedings of the 15th ACM SIGKDD international conference on Knowledge discovery and data mining, 279-288. Paris, France: ACM. doi:10.1145/1557019.1557055

        Bai, N., E. Bae, A. Aroonnual, A.K Bhunia, J.P. Robinson and E.D. Hirleman, 2010. Morphology characterization of bacterial colonies for predicting forward scattering patterns. Topical Meeting of Optical Society of America: Optical Sensors.

        Bai, N., Y. Tang, A. K. Bhunia, E. D. Hirleman, E. Bae. 2011. Characterization of optical properties of bacterial micro-colonies via the comprehensive morphology analyzer. Proceedings of SPIE, 8027: 80270C

        Rajwa, B., Dundar, M.M., Akova, F., Patsekin, V., Bae, E., Tang, Y., Dietz, J.E., Hirleman, E.D., Robinson, J.P., and Bhunia, A.K. 2011. Digital microbiology: detection and classification of unknown bacterial pathogens using a label-free laser light scatter-sensing system. Proceedings of SPIE, Vol.


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