John Patterson


John Patterson's Main Page

Research Program

There is increasing evidence that there is communication between the intestinal microbiota and epithelial and immune function. Research in my laboratory is currently focusing the influence of dietary additions and stressors on the intestinal microbial community structure and microbial interactions influencing anaerobic digestion of waste biomass and production of hydrogen and methane. In collaboration with others, we are addressing the influence of diet and stressors on interactions between the intestinal microbiota, the mucosal epithelium and the mucosal immune system.

Diet and stressor influence on intestinal microbota


Anaerobic intestinal bacteria affect food digestion and health of all animal species. With over 10 billion bacterial cells per gram of intestinal contents, there are more bacteria in animal bodies than there are animal cells. Alterations in the intestinal microbiota can influence animal health and human food safety. Thus it is important to determine environmental and dietary factors that alter the intestinal bacterial community structure. We have addressed the effect of diet (US vs EU), probiotics and prebiotics, and stressors (fasting and elevated temperature) on animal performance. We use classical microbial enumeration and Denaturing Gradient Gel Electrophoresis (DGGE) to determine changes in bacterial communigy structure. We have also shown that stressors influence attachment of salmonella to ileal tissues.

Microbial digestion of waste biomass

Microbial interactions influence fermentation products from intestinal substrates and from waste biomass. We are especially interested in the influence of interspecies hydrogen transfer reactions on microbial fermentation produces. We are also investigating maximizing hydrogen or methane production from waste biomass.

Research Techniques

  • Anaerobic chambers
  • Batch and continuous cultures
  • Enumeration of intestinal microbial populations
  • Analysis of Microbial Community Structure (DGGE)
  • Gas chromatography
  • Enzymatic assays

Selected Publications

Rose D.J., J.A. Patterson, and B.R. Hamaker. 2010. Structural differences among alkali-soluble arabinoxylans from maize (Zea mays), rice (Oryza sativa), and wheat (Triticum aestivum) brans influence human fecal fermentation profiles. J Agric Food Chem. 58(1):493-9.

Eicher, S. D., J. A. Patterson, and M. R. Rostagno. 2011. Salmonella enterica Serovar Dublin may be attenuated by supplementation with beta-glucan plus ascorbic acid in neonatal calves. Vet Immunol and Immunopathology. 142: 258–264.

Weedman, S. M., M.H. Rostagno, J.A. Patterson, I. Yoon, G. Fitzner, and S.D. Eicher. 2011. Yeast culture supplement during nursing and transport affects immunity and intestinal microbial ecology of weanling pigs. J of Anim Sci. 2011. 89: 6, 1908-1921.

Kaur, A. D. J. Rose, R. Rumpagaporn, J.A. Patterson, and B.R. Hamaker. 2011. In vitro batch fecal fermentation comparison of gas and short-chain fatty acid production using "slowly fermentable" dietary fibers. J of Food Sci. 76: 5, H137-H142.

Rumpagaporn, R., A. Kaur, O.H. Campanella, J. A. Patterson and B.R. Hamaker. 2012. Heat and pH stability of alkali-extractable corn arabinoxylan and its xylanase-hydrolyzate and their viscosity behavior. J Food Sci. 77(1):H23-H30.

Pinder, R.S., J.A. Patterson, C.A. O'Bryan, P.G. Crandall and S.C. Ricke. 2012. Dietary fiber content influences soluble carbohydrate levels in ruminal fluids. J. Environ. Sci and Health, Part B:Pesticides, Food Contam and Agric Wastes. 47(7) 710-717.

Jiang, W., R.S. Pinder, J.A. Patterson and S.C. Ricke. 2012. Sugar phosphorylation activity in ruminal acetogens. J. Environ. Sci. Health, Part A. 47:843-846.

Pinder,R.S. and J.A. Patterson. 2012. Glucose and hydrogen utilization by an acetogeinc bacterium isolated from ruminal contents. Agric. Food Anal. Bacteriol. 2(4):253-274.

Pinder, R.S. and J.A. Patterson. 2012. Growth of acetogenic bacteria in response to varying pH, acetate or carbohydrate concentration. Agric. Food Anal. Bacteriol. 3(1):6-16.

Kramer, R.A., L. Pelter, R. Branch and J.A. Patterson. 2012. Enhancing DDGS Value With Anaerobic Hydrogen Production. International Fuel Ethanol Workshop and Expo. June, 9-11. Mineapolis, MN.

Boccazzi, P. and J.A. Patterson 2013. Isolation and initial characterization of acetogenic ruminal bacteria resistant to acidic conditions. Agric. Food Anal. Bacteriol. 3: 129-144.

Park, J.H., M. Kim, O. Kang, J.A. Patterson and C. H. Kim. 2014. Short chain fatty acids induce both effector and regulatory T cells by suppression of histone deacetylases and regulation of the mTOR S6K pathway. Mucosal Immunol. (11 June, 2014) doi:10.1038/mi.2014.44.

Boccazzi, P. and J.A. Patterson. 2014. Batch culture characterization of acetogenesis in ruminal contents: Influence of acetogen inocula concentration and addition of 2-bromoethanesulfonic acid. Agric. Food Anal. Bacteriol. (Accepted).

Patterson, J.A. and S.C. Ricke. 2014. Effect of ethanol and methanol on growth of ruminal bacteria Selenomonas ruminantium and Butyrivibrio fibrisolvens. J. Envrion. Sci and Health, Part B: Pesticides, Food Contamin Agric Waste. (Accepted).

Kramer, R.A., L. Pelter and J.A. Patterson. 2014. New method to increase DDGS value and ethanol production efficiency. Int. Fuel Ethanol Workshop and Expo. June 9-12, 2014. Indianapolis, IN.