Optimizing Beam Current - SEM

What is beam current? Beam current is a measurement of the number of electrons in the electron beam.  In an SEM, beam current has three major impacts on your image: signal to noise (S/N), charging, and resolving power. No two samples are the same, so no single beam current is good for all samples!  Below is a guideline to help you optimize beam current for your sample. 

Low Signal to Noise (S/N)

  • Signal is low because there are not many electrons hitting the sample.
  • Compensate for low S/N by using longer pixel dwell times during image collection.
Decreased Charging
  • There are not many electrons hitting the sample, which reduces charging. 
Higher Maximum Resolution
  • Allows you to resolve smaller features because the size of the beam is smaller.

High Signal to Noise (S/N)

  • Many electrons hit the sample, producing a large amount of signal. 
  • The amount of noise does not change, so the S/N ratio improves.
Worsens Charging
  • There are a large number of electrons hitting the sample surface, which take longer to be grounded, especially by a non-conductive material. 
Lower Maximum Resolution
  • Resolution decreases when the size of the beam becomes larger than the features of interest. 

Sufficient Signal to Noise (S/N)

  • An optimal beam current produces sufficient S/N to produce quality images. 
  • While optimizing beam current, also experiment with pixel dwell times. Dwell time has a large impact on S/N too. 
Minimizes Charging
  • An optimal beam current produces little to no charging. 
  • Coating samples with a conductive material can help reduce charging too. 
Sufficient Resolution
  • An optimal beam current allows you to resolve features of interest. 
  • Resolving smaller features requires the use of a lower beam current. 

Low Signal to Noise (S/N)

  • Signal is low because there are not many electrons hitting the sample.
  • Compensate for low S/N by using longer pixel dwell times during image collection.
Decreased Charging
  • There are not many electrons hitting the sample, which reduces charging. 
Higher Maximum Resolution
  • Allows you to resolve smaller features because the size of the beam is smaller.

High Signal to Noise (S/N)

  • Many electrons hit the sample, producing a large amount of signal. 
  • The amount of noise does not change, so the S/N ratio improves.
Worsens Charging
  • There are a large number of electrons hitting the sample surface, which take longer to be grounded, especially by a non-conductive material. 
Lower Maximum Resolution
  • Resolution decreases when the size of the beam becomes larger than the features of interest. 

Sufficient Signal to Noise (S/N)

  • An optimal beam current produces sufficient S/N to produce quality images. 
  • While optimizing beam current, also experiment with pixel dwell times. Dwell time has a large impact on S/N too. 
Minimizes Charging
  • An optimal beam current produces little to no charging. 
  • Coating samples with a conductive material can help reduce charging too. 
Sufficient Resolution
  • An optimal beam current allows you to resolve features of interest. 
  • Resolving smaller features requires the use of a lower beam current. 

Paper imaged using 3.1pA.
Paper imaged using 0.8 nA.
Paper imaged using 13 nA.
Interested in learning more about optimizing SEM parameters?  Learn about pixel dwell time next!  Or use the side menu to navigate to another SEM training topic, or back to the home page. 

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On to Pixel Dwell Time!

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On to Pixel Dwell Time!
Please send any questions/comments/concerns regarding the content on this page to snbienz@purdue.edu.