Skip to Main Content

Researchers find alternate path for Listeria to sicken people

Purdue University scientists have found another pathway that Listeria uses to enter the bloodstream, suggesting that forms of the foodborne bacteria considered benign may be more dangerous than once thought.

About 600 million people around the world contract listeriosis each year, with 420,000 dying, according to the World Health Organization. In the U.S., 1,600 people are infected each year, causing 260 deaths, according to the U.S. Centers for Disease Control and Prevention. Pregnant women, newborn children, adults 65 and older and people with weakened immune systems are most at risk. The pathogen is found most often in deli meat, hot dogs, dairy products and produce.

To infect someone, the Listeria monocytogenes bacteria must cross the epithelial barrier, a wall of cells in the gut that generally protects the bloodstream from harmful pathogens. It has long been believed that a protein called Internalin A, found in many forms of Listeria, is required for breaking through that barrier.

Arun Bhunia, a Purdue food microbiology professor in the Department of Food Science, and his team fed Listeria to mice that have a non-functional receptor for the Internalin A protein. If Internalin A is required for Listeria monocytogenes bacteria to reach the bloodstream, these mice should not have been infected. But they were.

“As soon as we feed the mice this bacterium, it goes through the intestine and crosses this epithelial barrier and into the blood circulation, liver and spleen,” Bhunia said. “This suggests there is another way in which Listeria monocytogenes gets through these cells and into the bloodstream.”

Bhunia’s findings, reported in the journal Cell Host & Microbe, show Listeria adhesion protein (LAP) interacts with heat shock protein (HSP) in mice, then the epithelial cells move apart to give the bacterium access to the bloodstream. But that is just one path the bacteria might use to infect a host.

Since the Internalin A receptors are sandwiched between epithelial cells in humans, they are inaccessible to the Internalin A protein.

“When LAP interacts with HSP, those cells move apart, not only giving the bacterium access to the bloodstream, but also exposing the Internalin A receptors and allowing transport of Listeria monocytogenes into the bloodstream, which likely happens in humans,” Bhunia said. “Now that we know the mechanism, we can look at how to block this pathway to prevent the infection.”

The findings are also important because Listeria strains that have the defective or nonfunctional Internalin A protein haven’t been considered dangerous. But if the bacteria have another way through the gut and into the bloodstream regardless of Internalin A, that’s no longer true.

“We cannot just think that because most of these bacteria that are found in food are Internalin A mutants, we can tolerate it. We can still get infected,” said Rishi Drolia, a graduate research assistant in Bhunia’s lab.

Next, Bhunia’s team is working on development of a vaccine that blocks LAP, which could be a method for keeping Listeria from reaching the bloodstream. The Purdue AgSEED program, the U.S. Department of Agriculture and the Purdue Research Foundation supported this research. 

Listeria monocytogenes, shown in green in these microscope slides, has crossed the epithelial barrier of the intestines by a wild-type (WT) Listeria monocytogenes on the left and the Internalin A deficient (?inlA) L. monocytogenes on the right. In the center (lap—), the bacterium cannot cross the epithelial barrier in the mouse that has an Internalin A receptor deficiency. (Photo provided by Arun Bhunia) Listeria monocytogenes, shown in green in these microscope slides, has crossed the epithelial barrier of the intestines by a wild-type (WT) Listeria monocytogenes on the left and the Internalin A deficient (?inlA) L. monocytogenes on the right. In the center (lap—), the bacterium cannot cross the epithelial barrier in the mouse that has an Internalin A receptor deficiency. (Photo provided by Arun Bhunia)

Featured Stories

Yunmei Huang: Purdue’s Esri student of the year
Yunmei Huang: Purdue’s Esri student of the year

What if? It’s a question that drives innovation, and one that inspires Yunmei Huang, a PhD...

Read More
Dairy cows
Milk and motorsports: dairy’s lasting legacy in the Indy 500

Sunday, Hoosiers and racing fans from across the country gathered to watch the iconic Indy 500...

Read More
group of awardees
2025 Department of Biochemistry Spring Awards

The following awards, scholarships, and recognitions are presented by the department each Spring.

Read More
Jim Forney portrait
Professor Jim Forney Retires After 36 Years

Professor Jim Forney retired in Fall 2024 after 36 years at Purdue. During that time, he has...

Read More
powder being poured into a beaker
2024-25 Department of Biochemistry Publications

Publications associated with Department of Biochemistry faculty, postdoctoral researchers, and...

Read More
Carlos Corvalan, associate professor of food science and the project’s supervisor works on screen in lab at Purdue.
From lab to table: Purdue Food Science research predicts texture with machine learning

The creaminess of custard. The fizz of foam. The slurpability of soup. Texture is just as...

Read More
To Top